BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is upregulated and it recruits and activates inflammatory cells in murine lupus nephritis (LN). METHODS: Clinical outcomes of children with LN were examined in relation to glomerular expression of MCP-1 and macrophage infiltration, as determined by immunohistochemical staining of renal biopsy sections with MCP-1 and CD68. Sections were analysed using a modified histological score (H-score; maximum of 300) based on both percentage of positively stained cells and intensity of staining. RESULTS: Renal biopsies were examined from 34 children [27 (79%) female] aged 7.7-17.3 (median 13.7) years with 50% ISN/RPS Class IV LN. Renal dysfunction and proteinuria at follow-up of 2.2-15.4 (median 6.5) years were analysed with estimated glomerular filtration rates (eGFR) of 11.2-124.1 (median 93.6) ml/min/1.73 m(2) and urine albumin:creatinine ratios of 1-535 (median 63) mg/mmol. There was a correlation between glomerular expression of MCP-1 and CD68 (r = 0.98, P = 0.04; median modified H-score of 219.7 and 230.8, respectively). Patients with Class III and IV LN had increased glomerular expression of both MCP-1 and PGM1 compared to the other classes (P = 0.01) with Class IV-G LN patients having the most glomerular expression of MCP-1 (median of 227.3) and PGM1 (median of 237.5) and the worst renal prognosis (with proteinuria and reduced eGFR). CONCLUSIONS: There is a correlation between glomerular expression of MCP-1 and PGM1 and worsening renal prognosis in paediatric LN. Larger prospective studies of paediatric LN are required to further evaluate MCP-1 and other markers of disease progression.
BACKGROUND:Monocyte chemoattractant protein-1 (MCP-1) is upregulated and it recruits and activates inflammatory cells in murinelupus nephritis (LN). METHODS: Clinical outcomes of children with LN were examined in relation to glomerular expression of MCP-1 and macrophage infiltration, as determined by immunohistochemical staining of renal biopsy sections with MCP-1 and CD68. Sections were analysed using a modified histological score (H-score; maximum of 300) based on both percentage of positively stained cells and intensity of staining. RESULTS: Renal biopsies were examined from 34 children [27 (79%) female] aged 7.7-17.3 (median 13.7) years with 50% ISN/RPS Class IV LN. Renal dysfunction and proteinuria at follow-up of 2.2-15.4 (median 6.5) years were analysed with estimated glomerular filtration rates (eGFR) of 11.2-124.1 (median 93.6) ml/min/1.73 m(2) and urine albumin:creatinine ratios of 1-535 (median 63) mg/mmol. There was a correlation between glomerular expression of MCP-1 and CD68 (r = 0.98, P = 0.04; median modified H-score of 219.7 and 230.8, respectively). Patients with Class III and IV LN had increased glomerular expression of both MCP-1 and PGM1 compared to the other classes (P = 0.01) with Class IV-G LN patients having the most glomerular expression of MCP-1 (median of 227.3) and PGM1 (median of 237.5) and the worst renal prognosis (with proteinuria and reduced eGFR). CONCLUSIONS: There is a correlation between glomerular expression of MCP-1 and PGM1 and worsening renal prognosis in paediatric LN. Larger prospective studies of paediatric LN are required to further evaluate MCP-1 and other markers of disease progression.
Authors: William E O'Gorman; Elena W Y Hsieh; Erica S Savig; Pier Federico Gherardini; Joseph D Hernandez; Leo Hansmann; Imelda M Balboni; Paul J Utz; Sean C Bendall; Wendy J Fantl; David B Lewis; Garry P Nolan; Mark M Davis Journal: J Allergy Clin Immunol Date: 2015-05-30 Impact factor: 10.793
Authors: I Parodis; H Ding; A Zickert; L Arnaud; A Larsson; E Svenungsson; C Mohan; I Gunnarsson Journal: Scand J Rheumatol Date: 2016-12-15 Impact factor: 3.641
Authors: Paula S Ramos; James C Oates; Diane L Kamen; Adrienne H Williams; Patrick M Gaffney; Jennifer A Kelly; Kenneth M Kaufman; Robert P Kimberly; Timothy B Niewold; Chaim O Jacob; Betty P Tsao; Graciela S Alarcón; Elizabeth E Brown; Jeffrey C Edberg; Michelle A Petri; Rosalind Ramsey-Goldman; John D Reveille; Luis M Vilá; Judith A James; Joel M Guthridge; Joan T Merrill; Susan A Boackle; Barry I Freedman; R Hal Scofield; Anne M Stevens; Timothy J Vyse; Lindsey A Criswell; Kathy L Moser; Marta E Alarcón-Riquelme; Carl D Langefeld; John B Harley; Gary S Gilkeson Journal: J Rheumatol Date: 2013-05-01 Impact factor: 4.666