Jin-Fang Zheng1, Li-Jian Liang. 1. Department of Hepatobiliary Surgery, the People's Hospital of Hainan Province, Hainan Province, China. zhengjf2000@hotmail.com
Abstract
AIM: To investigate the malignant potential of hepatic stem cells derived from the bone marrow stromal cells (BMSCs) in a mouse model of chemical hepatocarcino-genesis. METHODS: BMSCs from male BALB/c mice were harvested and cultured, then transplanted into female syngenic BALB/c mice via portal vein. Hepato-carcinogenesis was induced by 6 months of treatment with diethylnitrosamine (DEN). Six months later, the liver was removed from each treated mouse and evaluated by immunohistochemistry and fluorescence in situ hybridization (FISH). RESULTS: Twenty-six percent of recipient mice survived and developed multiple hepatocellular carcinomas (HCCs). Immunohistochemically, HCC expressed placental form of glutathione-S-transferase (GST-P) and alpha-fetoprotein, but did not express cytokeratin 19. Y chromosome positive hepatocytes were detected by fluorescent in situ hybridization (FISH) in the liver of mice treated with DEN after BMSCs transplantation while no such hepatocytes were identified in the liver of mice not treated with DEN. No HCC was positive for the Y chromosome by FISH. CONCLUSION: Hepatic stem cells derived from the bone marrow stromal cells have a low malignant potential in our mouse model of chemical hepatocarcingenesis.
AIM: To investigate the malignant potential of hepatic stem cells derived from the bone marrow stromal cells (BMSCs) in a mouse model of chemical hepatocarcino-genesis. METHODS: BMSCs from male BALB/c mice were harvested and cultured, then transplanted into female syngenic BALB/c mice via portal vein. Hepato-carcinogenesis was induced by 6 months of treatment with diethylnitrosamine (DEN). Six months later, the liver was removed from each treated mouse and evaluated by immunohistochemistry and fluorescence in situ hybridization (FISH). RESULTS: Twenty-six percent of recipient mice survived and developed multiple hepatocellular carcinomas (HCCs). Immunohistochemically, HCC expressed placental form of glutathione-S-transferase (GST-P) and alpha-fetoprotein, but did not express cytokeratin 19. Y chromosome positive hepatocytes were detected by fluorescent in situ hybridization (FISH) in the liver of mice treated with DEN after BMSCs transplantation while no such hepatocytes were identified in the liver of mice not treated with DEN. No HCC was positive for the Y chromosome by FISH. CONCLUSION: Hepatic stem cells derived from the bone marrow stromal cells have a low malignant potential in our mouse model of chemical hepatocarcingenesis.
Authors: B E Petersen; W C Bowen; K D Patrene; W M Mars; A K Sullivan; N Murase; S S Boggs; J S Greenberger; J P Goff Journal: Science Date: 1999-05-14 Impact factor: 47.728
Authors: Yoon-Young Jang; Michael I Collector; Stephen B Baylin; Anna Mae Diehl; Saul J Sharkis Journal: Nat Cell Biol Date: 2004-05-09 Impact factor: 28.824
Authors: Ju-Seog Lee; Jeonghoon Heo; Louis Libbrecht; In-Sun Chu; Pal Kaposi-Novak; Diego F Calvisi; Arsen Mikaelyan; Lewis R Roberts; Anthony J Demetris; Zongtang Sun; Frederik Nevens; Tania Roskams; Snorri S Thorgeirsson Journal: Nat Med Date: 2006-03-12 Impact factor: 53.440
Authors: Alexander V Kofman; Glyn Morgan; Adam Kirschenbaum; Jon Osbeck; Mehboob Hussain; Scott Swenson; Neil D Theise Journal: Hepatology Date: 2005-06 Impact factor: 17.425
Authors: Pamela Vig; Francesco P Russo; Robert J Edwards; Paul J Tadrous; Nicholas A Wright; Howard C Thomas; Malcolm R Alison; Stuart J Forbes Journal: Hepatology Date: 2006-02 Impact factor: 17.425