OBJECTIVE: To explore the effects of ageing on the viability of bioengineered striated muscle tissue in vivo, and if this viability can be enhanced by concurrent neovascularization, as its utility for the treatment of stress urinary incontinence (SUI) might be reduced if muscle cells are derived from old patients. MATERIALS AND METHODS: Myoblasts were obtained and expanded in culture from young (2 weeks), mature (3 months) and old (24 months) mice, and were engineered to express vascular endothelial growth factor (VEGF) to stimulate neovascularization. Myoblasts were injected subcutaneously into male nude mice and after 2 and 4 weeks, the engineered muscle tissues were harvested. RESULTS: Bioengineered muscle tissues were formed in all groups, but the engineered muscles formed by myoblasts from old mice were smaller and less contractile. However, the bioengineered muscles expressing VEGF had a greater mass and better contractility in all age groups. CONCLUSION: This pilot study showed that there was an age-related decline in the size and function of bioengineered muscle; however, there was an improvement in volume and function when the muscle cells were expressing VEGF.
OBJECTIVE: To explore the effects of ageing on the viability of bioengineered striated muscle tissue in vivo, and if this viability can be enhanced by concurrent neovascularization, as its utility for the treatment of stress urinary incontinence (SUI) might be reduced if muscle cells are derived from old patients. MATERIALS AND METHODS: Myoblasts were obtained and expanded in culture from young (2 weeks), mature (3 months) and old (24 months) mice, and were engineered to express vascular endothelial growth factor (VEGF) to stimulate neovascularization. Myoblasts were injected subcutaneously into male nude mice and after 2 and 4 weeks, the engineered muscle tissues were harvested. RESULTS: Bioengineered muscle tissues were formed in all groups, but the engineered muscles formed by myoblasts from old mice were smaller and less contractile. However, the bioengineered muscles expressing VEGF had a greater mass and better contractility in all age groups. CONCLUSION: This pilot study showed that there was an age-related decline in the size and function of bioengineered muscle; however, there was an improvement in volume and function when the muscle cells were expressing VEGF.
Authors: Bridget M Deasy; Joseph M Feduska; Thomas R Payne; Yong Li; Fabrisia Ambrosio; Johnny Huard Journal: Mol Ther Date: 2009-07-14 Impact factor: 11.454
Authors: Deana Haralampieva; Souzan Salemi; Thomas Betzel; Ivana Dinulovic; Stefanie D Krämer; Roger Schibli; Tullio Sulser; Christoph Handschin; Simon M Ametamey; Daniel Eberli Journal: Stem Cells Int Date: 2018-01-21 Impact factor: 5.443
Authors: Deana Haralampieva; Souzan Salemi; Ivana Dinulovic; Tullio Sulser; Simon M Ametamey; Christoph Handschin; Daniel Eberli Journal: Cell Transplant Date: 2017-02-03 Impact factor: 4.064
Authors: Deana Haralampieva; Thomas Betzel; Ivana Dinulovic; Souzan Salemi; Meline Stoelting; Stefanie D Krämer; Roger Schibli; Tullio Sulser; Christoph Handschin; Daniel Eberli; Simon M Ametamey Journal: J Nucl Med Date: 2016-05-19 Impact factor: 10.057
Authors: Shukui Zhou; Kaile Zhang; Anthony Atala; Oula Khoury; Sean V Murphy; Weixin Zhao; Qiang Fu Journal: Stem Cells Int Date: 2016-01-10 Impact factor: 5.443