Literature DB >> 18487404

Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome.

Wei Sun1, Shifeng Wang, Roy Curtiss.   

Abstract

An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines lambda Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.

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Year:  2008        PMID： 18487404      PMCID： PMC2446500          DOI： 10.1128/AEM.00940-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

1.  Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.

Authors:  K C Murphy
Journal:  J Bacteriol       Date:  1998-04       Impact factor: 3.490

2.  Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression.

Authors:  R A Edwards; L H Keller; D M Schifferli
Journal:  Gene       Date:  1998-01-30       Impact factor: 3.688

3.  RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague.

Authors:  Jason S Cathelyn; Seth D Crosby; Wyndham W Lathem; William E Goldman; Virginia L Miller
Journal:  Proc Natl Acad Sci U S A       Date:  2006-08-28       Impact factor: 11.205

4.  A dam mutant of Yersinia pestis is attenuated and induces protection against plague.

Authors:  Victoria L Robinson; Petra C F Oyston; Richard W Titball
Journal:  FEMS Microbiol Lett       Date:  2005-09-15       Impact factor: 2.742

5.  Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Authors:  L M Guzman; D Belin; M J Carson; J Beckwith
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

6.  Rapid method for the construction of Salmonella enterica Serovar Typhimurium vaccine carrier strains.

Authors:  Mohamed I Husseiny; Michael Hensel
Journal:  Infect Immun       Date:  2005-03       Impact factor: 3.441

7.  An ABC transporter system of Yersinia pestis allows utilization of chelated iron by Escherichia coli SAB11.

Authors:  S W Bearden; T M Staggs; R D Perry
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

8.  New suicide vector for gene replacement in yersiniae and other gram-negative bacteria.

Authors:  E Skrzypek; P L Haddix; G V Plano; S C Straley
Journal:  Plasmid       Date:  1993-03       Impact factor: 3.466

9.  A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica.

Authors:  K Kaniga; I Delor; G R Cornelis
Journal:  Gene       Date:  1991-12-20       Impact factor: 3.688

10.  A putative DNA adenine methyltransferase is involved in Yersinia pseudotuberculosis pathogenicity.

Authors:  Flavie Pouillot; Corinne Fayolle; Elisabeth Carniel
Journal:  Microbiology (Reading)       Date:  2007-08       Impact factor: 2.777
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  32 in total

1.  A method for generating precise gene deletions and insertions in Escherichia coli.

Authors:  Qi-Ming Zhou; Dong-Jie Fan; Jiang-Bi Xie; Chuan-Peng Liu; Jun-Mei Zhou
Journal:  World J Microbiol Biotechnol       Date:  2010-01-08       Impact factor: 3.312

Review 2.  Whole-genome sequencing in outbreak analysis.

Authors:  Carol A Gilchrist; Stephen D Turner; Margaret F Riley; William A Petri; Erik L Hewlett
Journal:  Clin Microbiol Rev       Date:  2015-07       Impact factor: 26.132

3.  Knockout and pullout recombineering for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei.

Authors:  Yun Kang; Michael H Norris; Bruce A Wilcox; Apichai Tuanyok; Paul S Keim; Tung T Hoang
Journal:  Nat Protoc       Date:  2011-07-07       Impact factor: 13.491

4.  Regulated delayed expression of rfc enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica vaccines.

Authors:  Qingke Kong; Qing Liu; Angela M Jansen; Roy Curtiss
Journal:  Vaccine       Date:  2010-07-03       Impact factor: 3.641

5.  Yersinia pestis with regulated delayed attenuation as a vaccine candidate to induce protective immunity against plague.

Authors:  Wei Sun; Kenneth L Roland; Xiaoying Kuang; Christine G Branger; Roy Curtiss
Journal:  Infect Immun       Date:  2010-01-19       Impact factor: 3.441

6.  Amino acid substitutions in LcrV at putative sites of interaction with Toll-like receptor 2 do not affect the virulence of Yersinia pestis.

Authors:  Wei Sun; Roy Curtiss
Journal:  Microb Pathog       Date:  2012-07-24       Impact factor: 3.738

7.  Live recombinant Salmonella Typhi vaccines constructed to investigate the role of rpoS in eliciting immunity to a heterologous antigen.

Authors:  Huoying Shi; Javier Santander; Karen E Brenneman; Soo-Young Wanda; Shifeng Wang; Patti Senechal; Wei Sun; Kenneth L Roland; Roy Curtiss
Journal:  PLoS One       Date:  2010-06-18       Impact factor: 3.240

8.  Pathogenicity of Yersinia pestis synthesis of 1-dephosphorylated lipid A.

Authors:  Wei Sun; David A Six; C Michael Reynolds; Hak Suk Chung; Christian R H Raetz; Roy Curtiss
Journal:  Infect Immun       Date:  2013-01-28       Impact factor: 3.441

9.  A Yersinia pestis guaBA mutant is attenuated in virulence and provides protection against plague in a mouse model of infection.

Authors:  Petra C F Oyston; Gabriela Mellado-Sanchez; Marcela F Pasetti; James P Nataro; Richard W Titball; Helen S Atkins
Journal:  Microb Pathog       Date:  2010-01-22       Impact factor: 3.738

10.  The role of relA and spoT in Yersinia pestis KIM5 pathogenicity.

Authors:  Wei Sun; Kenneth L Roland; Christine G Branger; Xiaoying Kuang; Roy Curtiss
Journal:  PLoS One       Date:  2009-08-24       Impact factor: 3.240
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