OBJECTIVE: Data on trophoblast particles in peripheral maternal circulation are rare. Applying a new enrichment technique, Oncoquick, we evaluated the number of trophoblast particles in maternal blood in preeclamptic and non-preeclamptic pregnancies. METHODS: After isolation of trophoblast particles using Oncoquick, trophoblast particles were detected by immunocytochemistry, applying a monoclonal pan cytokeratin (CK) antibody. Fetal origin of the detected particles was verified by XY- fish analysis in women delivering male infants. RESULTS: Trophoblast particles were identified in 25/52 (48%) of preeclamptic and 1/20 (5%) of non-preeclamptic pregnancies. CONCLUSION: The number of trophoblast particles in peripheral maternal blood of preeclamptic pregnancies was significantly higher than in non-preeclamptic pregnancies.
OBJECTIVE: Data on trophoblast particles in peripheral maternal circulation are rare. Applying a new enrichment technique, Oncoquick, we evaluated the number of trophoblast particles in maternal blood in preeclamptic and non-preeclamptic pregnancies. METHODS: After isolation of trophoblast particles using Oncoquick, trophoblast particles were detected by immunocytochemistry, applying a monoclonal pan cytokeratin (CK) antibody. Fetal origin of the detected particles was verified by XY- fish analysis in women delivering male infants. RESULTS: Trophoblast particles were identified in 25/52 (48%) of preeclamptic and 1/20 (5%) of non-preeclamptic pregnancies. CONCLUSION: The number of trophoblast particles in peripheral maternal blood of preeclamptic pregnancies was significantly higher than in non-preeclamptic pregnancies.
Authors: Lorenz Kuessel; Harald Zeisler; Robin Ristl; Julia Binder; Petra Pateisky; Maximilian Schmid; Julian Marschalek; Thomas Perkmann; Helmuth Haslacher; Heinrich Husslein Journal: BMC Pregnancy Childbirth Date: 2016-11-03 Impact factor: 3.007
Authors: Benjamin S Umezuluike; Chidebe C Anikwe; Oluomachi C Nnachi; Bobbie C A Iwe; Chinedu C Ifemelumma; Ikechukwu B O Dimejesi Journal: Heliyon Date: 2021-11-26