| Literature DB >> 18482605 |
Raju Khan1, Ajeet Kaushik, Pratima R Solanki, Anees A Ansari, Manoj K Pandey, B D Malhotra.
Abstract
Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41x10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K(m)) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples.Entities:
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Year: 2008 PMID： 18482605 DOI： 10.1016/j.aca.2008.04.010
Source DB: PubMed Journal: Anal Chim Acta ISSN： 0003-2670 Impact factor: 6.558