Literature DB >> 1847911

Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.

T Sugahara1, Y Konno, H Ohta, K Ito, J Kaneko, Y Kamio, K Izaki.   

Abstract

Two alkaline phosphatases were extracted from the membranes of Bacillus subtilis 168 stationary-phase cells and purified as homogeneous proteins by hydroxyapatite column chromatography. Alkaline phosphatases I and II differed in several properties such as subunit molecular weight, substrate specificity, thermostability, Km, pH stability, and peptide maps.

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Year:  1991        PMID： 1847911      PMCID： PMC207338          DOI： 10.1128/jb.173.5.1824-1826.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  8 in total

1.  Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis.

Authors:  F M Hulett; C Bookstein; K Jensen
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

2.  Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis.

Authors:  K Yamane; B Maruo
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

3.  Purification, subunit structure and properties of two repressible phosphohydrolases of Bacillus subtilis.

Authors:  J C Le Hégarat; C Anagnostopoulos
Journal:  Eur J Biochem       Date:  1973-11-15

4.  Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors:  J L Hedrick; A J Smith
Journal:  Arch Biochem Biophys       Date:  1968-07       Impact factor: 4.013

5.  Radioiodination of microgram quantities of ribosomal proteins from polyacrylamide gels.

Authors:  D R Tolan; J M Lambert; G Boileau; T G Fanning; J W Kenny; A Vassos; R R Traut
Journal:  Anal Biochem       Date:  1980-03-15       Impact factor: 3.365

6.  Sporulation in Bacillus subtilis 168. Control of synthesis of alkaline phosphatase.

Authors:  W D Grant
Journal:  J Gen Microbiol       Date:  1974-06

7.  Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells.

Authors:  A R Glenn; J Mandelstam
Journal:  Biochem J       Date:  1971-06       Impact factor: 3.857

8.  Microbiological aspects of wood chip storage in tropical environments.

Authors:  H Greaves
Journal:  Aust J Biol Sci       Date:  1975-06
  8 in total
  2 in total

1.  Duplicate isochorismate synthase genes of Bacillus subtilis: regulation and involvement in the biosyntheses of menaquinone and 2,3-dihydroxybenzoate.

Authors:  B M Rowland; H W Taber
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

2.  Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis.

Authors:  Lia M Godinho; Isabel de Sá-Nogueira
Journal:  FEBS J       Date:  2011-06-02       Impact factor: 5.542
  2 in total

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