Literature DB >> 1847723

Listeria monocytogenes mutants lacking phosphatidylinositol-specific phospholipase C are avirulent.

A Camilli1, H Goldfine, D A Portnoy.   

Abstract

A number of bacterial species secrete phosphatidylinositol-specific phospholipase C (PI-PLC). In this report, we show that the facultative intracellular bacterial pathogen, Listeria monocytogenes, contains a gene, plcA, predicting a polypeptide with 31% amino acid identity to a Bacillus thuringiensis PI-PLC. Accordingly, L. monocytogenes secretes PI-PLC activity, while a mutant with a transposon insertion in plcA lacks detectable PI-PLC activity. In addition, expression of plcA in B. subtilis resulted in secretion of PI-PLC activity. The L. monocytogenes PI-PLC-defective mutant was three logs less virulent for mice and failed to grow in host tissues. The mutant was also defective for in vitro growth in mouse peritoneal macrophages. These results strongly suggest that PI-PLC is an essential determinant of L. monocytogenes pathogenesis. Whether the PI-PLC acts on a bacterial or host substrate remains to be determined.

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Year:  1991        PMID： 1847723      PMCID： PMC2118838          DOI： 10.1084/jem.173.3.751

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  17 in total

1.  Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread.

Authors:  A N Sun; A Camilli; D A Portnoy
Journal:  Infect Immun       Date:  1990-11       Impact factor: 3.441

Review 2.  The glycosyl-phosphatidylinositol anchor of membrane proteins.

Authors:  M G Low
Journal:  Biochim Biophys Acta       Date:  1989-12-06

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Authors:  S Falkow
Journal:  Rev Infect Dis       Date:  1988 Jul-Aug

4.  Rapid and sensitive protein similarity searches.

Authors:  D J Lipman; W R Pearson
Journal:  Science       Date:  1985-03-22       Impact factor: 47.728

5.  Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation.

Authors:  J Mengaud; M F Vicente; P Cossart
Journal:  Infect Immun       Date:  1989-12       Impact factor: 3.441

6.  Phospholipids of Listeria monocytogenes.

Authors:  N Kosaric; K K Carroll
Journal:  Biochim Biophys Acta       Date:  1971-09-01

7.  In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2.

Authors:  J L Gaillard; P Berche; J Mounier; S Richard; P Sansonetti
Journal:  Infect Immun       Date:  1987-11       Impact factor: 3.441

8.  Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes.

Authors:  L G Tilney; D A Portnoy
Journal:  J Cell Biol       Date:  1989-10       Impact factor: 10.539

9.  Role of hemolysin for the intracellular growth of Listeria monocytogenes.

Authors:  D A Portnoy; P S Jacks; D J Hinrichs
Journal:  J Exp Med       Date:  1988-04-01       Impact factor: 14.307

10.  Gamma interferon limits access of Listeria monocytogenes to the macrophage cytoplasm.

Authors:  D A Portnoy; R D Schreiber; P Connelly; L G Tilney
Journal:  J Exp Med       Date:  1989-12-01       Impact factor: 14.307
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  73 in total

1.  Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in Listeria monocytogenes.

Authors:  P D Cotter; N Emerson; C G Gahan; C Hill
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

Review 2.  Molecular determinants of Listeria monocytogenes pathogenesis.

Authors:  D A Portnoy; T Chakraborty; W Goebel; P Cossart
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441

3.  Cytotoxic-T-lymphocyte responses to epitopes of listeriolysin O and p60 following infection with Listeria monocytogenes.

Authors:  H G Bouwer; D J Hinrichs
Journal:  Infect Immun       Date:  1996-07       Impact factor: 3.441

4.  Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles.

Authors:  Carolina Coelho; Lisa Brown; Maria Maryam; Raghav Vij; Daniel F Q Smith; Meagan C Burnet; Jennifer E Kyle; Heino M Heyman; Jasmine Ramirez; Rafael Prados-Rosales; Gregoire Lauvau; Ernesto S Nakayasu; Nathan R Brady; Anne Hamacher-Brady; Isabelle Coppens; Arturo Casadevall
Journal:  J Biol Chem       Date:  2018-11-30       Impact factor: 5.157

Review 5.  Listeria pathogenesis and molecular virulence determinants.

Authors:  J A Vázquez-Boland; M Kuhn; P Berche; T Chakraborty; G Domínguez-Bernal; W Goebel; B González-Zorn; J Wehland; J Kreft
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

6.  The virulence gene cluster of Listeria monocytogenes is also present in Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species.

Authors:  E Gouin; J Mengaud; P Cossart
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

7.  The two distinct phospholipases C of Listeria monocytogenes have overlapping roles in escape from a vacuole and cell-to-cell spread.

Authors:  G A Smith; H Marquis; S Jones; N C Johnston; D A Portnoy; H Goldfine
Journal:  Infect Immun       Date:  1995-11       Impact factor: 3.441

8.  Membrane permeabilization by Listeria monocytogenes phosphatidylinositol-specific phospholipase C is independent of phospholipid hydrolysis and cooperative with listeriolysin O.

Authors:  H Goldfine; C Knob; D Alford; J Bentz
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

9.  Roles of Listeria monocytogenes virulence factors in survival: virulence factors distinct from listeriolysin are needed for the organism to survive an early neutrophil-mediated host defense mechanism.

Authors:  J W Conlan; R J North
Journal:  Infect Immun       Date:  1992-03       Impact factor: 3.441

10.  Pathogenicity and immunogenicity of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread.

Authors:  R A Barry; H G Bouwer; D A Portnoy; D J Hinrichs
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441
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