A K Sood1, Z Bahrani-Mostafavi, J Stoerker, I K Stone. 1. Department of Obstetrics and Gynecology University of Florida College of Medicine P.O. Box 100294 Gainesville, FL 32610-0294, USA.
Abstract
OBJECTIVE: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes. METHODS: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers. RESULTS: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters. CONCLUSIONS: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.
OBJECTIVE: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes. METHODS: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers. RESULTS: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters. CONCLUSIONS: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.
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