Literature DB >> 18473945

Characterization of fluoroalcohols-induced intermediates of Mucor miehei lipase at low pH.

Sadaf Fatima1, Aseem Mishra, Priyankar Sen, Rizwan Hasan Khan.   

Abstract

We have previously characterized an acid-unfolded (U(A)) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with beta-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. alpha-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.

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Year:  2008        PMID: 18473945     DOI: 10.2174/092986608784246425

Source DB:  PubMed          Journal:  Protein Pept Lett        ISSN: 0929-8665            Impact factor:   1.890


  1 in total

1.  Insights into Kinetics of Agitation-Induced Aggregation of Hen Lysozyme under Heat and Acidic Conditions from Various Spectroscopic Methods.

Authors:  Ali Chaari; Christine Fahy; Alexandre Chevillot-Biraud; Mohamed Rholam
Journal:  PLoS One       Date:  2015-11-16       Impact factor: 3.240

  1 in total

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