AIM: To identify the cholecystokinin (CCK)-A receptors (CCK-AR) on the guniea pig gallbladder interstitial cells of cajal (ICC) and to study CCK-8 induced gallbladder muscle strip contractions through the CCK-AR. METHODS: The existence of CCK-AR was examined by immunohistofluorescence on sectioned tissue and cultured cells. In vitro contractile response of guinea pig gallbladder muscle strips and the strips with ICC removed were also studied with CCK-8 receptors added. RESULTS: In tissue sections, intensely CCKAR-immunoreactive interstitial cells were found mainly in the muscular layers. In cultured cell sections, distinctive double staining of C-kit and CCK-AR ICCs were found. When we removed the ICC of the gallbladder, CCK-8 induced muscle strip contraction dose response curve significantly shifted to the right. CONCLUSION: We proved that both the existence of CCK-AR on the guinea pig gallbladder ICC and CCK evoked contraction are mediated through direct action on CCK-AR on the gallbladder ICC.
AIM: To identify the cholecystokinin (CCK)-A receptors (CCK-AR) on the guniea pig gallbladder interstitial cells of cajal (ICC) and to study CCK-8 induced gallbladder muscle strip contractions through the CCK-AR. METHODS: The existence of CCK-AR was examined by immunohistofluorescence on sectioned tissue and cultured cells. In vitro contractile response of guinea pig gallbladder muscle strips and the strips with ICC removed were also studied with CCK-8 receptors added. RESULTS: In tissue sections, intensely CCKAR-immunoreactive interstitial cells were found mainly in the muscular layers. In cultured cell sections, distinctive double staining of C-kit and CCK-AR ICCs were found. When we removed the ICC of the gallbladder, CCK-8 induced muscle strip contraction dose response curve significantly shifted to the right. CONCLUSION: We proved that both the existence of CCK-AR on the guinea pig gallbladder ICC and CCK evoked contraction are mediated through direct action on CCK-AR on the gallbladder ICC.
Authors: D Gully; D Fréhel; C Marcy; A Spinazzé; L Lespy; G Neliat; J P Maffrand; G Le Fur Journal: Eur J Pharmacol Date: 1993-02-23 Impact factor: 4.432
Authors: Concepción Junquera; Carmen Martínez-Ciriano; Tomás Castiella; Pedro Serrano; María Jesús Azanza; Santiago Ramón y Cajal Junquera Journal: J Cell Mol Med Date: 2007 Jul-Aug Impact factor: 5.310
Authors: Andrzej Matyja; Krzysztof Gil; Artur Pasternak; Krystyna Sztefko; Mariusz Gajda; Krzysztof A Tomaszewski; Maciej Matyja; Jerzy A Walocha; Jan Kulig; Piotr Thor Journal: J Cell Mol Med Date: 2013-04-04 Impact factor: 5.310