| Literature DB >> 18471258 |
Kai-Ming Chan1, Sai-Chuen Fu, Yim-Ping Wong, Wun-Chun Hui, Yau-Chuk Cheuk, Margaret Wan-Nar Wong.
Abstract
Transforming growth factor beta (TGF-beta) plays active roles in tendon healing. However, the differential effects of TGF-beta isoforms on tendon healing have not been investigated. In cultured tendon fibroblasts, we tested the effects of TGF-beta1, beta2, and beta3 on the mRNA levels of COL1A1 and COL3A1 by quantitative real-time polymerase chain reaction. We also investigated the expression of TGF-beta isoforms, TGF-beta receptors, procollagen Type I and Type III in a rat model of tendon healing. We found that TGF-beta3 exhibited the highest potency in stimulating COL1A1 and COL3A1. TGF-beta1 exerted antagonistic effects to TGF-beta2 and beta3. All TGF-beta isoforms and procollagen Type I were confined to the edges of the healing tendon at day 28 postinjury. Our results indicated that interaction of TGF-beta isoforms exist in the regulation of collagen synthesis in tendon fibroblasts. Their effects may be further complicated by uneven spatial distribution of TGF-beta and TGF-beta receptors in healing tendons.Entities:
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Year: 2008 PMID： 18471258 DOI： 10.1111/j.1524-475X.2008.00379.x
Source DB: PubMed Journal: Wound Repair Regen ISSN： 1067-1927 Impact factor: 3.617