Literature DB >> 18469458

Live single-cell molecular analysis by video-mass spectrometry.

Naohiro Tsuyama1, Hajime Mizuno, Emi Tokunaga, Tsutomu Masujima.   

Abstract

The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.

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Year:  2008        PMID： 18469458     DOI： 10.2116/analsci.24.559

Source DB:  PubMed          Journal:  Anal Sci        ISSN： 0910-6340            Impact factor:   2.081


  11 in total

1.  Direct metabolomics for plant cells by live single-cell mass spectrometry.

Authors:  Takashi Fujii; Shuichi Matsuda; Mónica Lorenzo Tejedor; Tsuyoshi Esaki; Iwao Sakane; Hajime Mizuno; Naohiro Tsuyama; Tsutomu Masujima
Journal:  Nat Protoc       Date:  2015-08-27       Impact factor: 13.491

Review 2.  In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells.

Authors:  Yousef Gholipour; Rosa Erra-Balsells; Hiroshi Nonami
Journal:  Mass Spectrom (Tokyo)       Date:  2012-07-05

Review 3.  Single cell optical transfection.

Authors:  David J Stevenson; Frank J Gunn-Moore; Paul Campbell; Kishan Dholakia
Journal:  J R Soc Interface       Date:  2010-01-11       Impact factor: 4.118

4.  Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells.

Authors:  Shawna J Standke; Devon H Colby; Ryan C Bensen; Anthony W G Burgett; Zhibo Yang
Journal:  J Vis Exp       Date:  2019-06-21       Impact factor: 1.355

5.  Redesigning the T-probe for mass spectrometry analysis of online lysis of non-adherent single cells.

Authors:  Yanlin Zhu; Renmeng Liu; Zhibo Yang
Journal:  Anal Chim Acta       Date:  2019-07-31       Impact factor: 6.558

Review 6.  Global discovery of protein kinases and other nucleotide-binding proteins by mass spectrometry.

Authors:  Yongsheng Xiao; Yinsheng Wang
Journal:  Mass Spectrom Rev       Date:  2014-11-05       Impact factor: 10.946

Review 7.  Chemical analysis of single cells.

Authors:  Yuqing Lin; Raphaël Trouillon; Gulnara Safina; Andrew G Ewing
Journal:  Anal Chem       Date:  2011-04-28       Impact factor: 6.986

8.  Mass Spectrometry Measurement of Single Suspended Cells Using a Combined Cell Manipulation System and a Single-Probe Device.

Authors:  Shawna J Standke; Devon H Colby; Ryan C Bensen; Anthony W G Burgett; Zhibo Yang
Journal:  Anal Chem       Date:  2019-01-18       Impact factor: 6.986

9.  Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-Bond Positions in Lipids at the Single-Cell Level.

Authors:  Yanlin Zhu; Wenhua Wang; Zhibo Yang
Journal:  Anal Chem       Date:  2020-07-27       Impact factor: 6.986

10.  Patch clamp electrophysiology and capillary electrophoresis-mass spectrometry metabolomics for single cell characterization.

Authors:  Jordan T Aerts; Kathleen R Louis; Shane R Crandall; Gubbi Govindaiah; Charles L Cox; Jonathan V Sweedler
Journal:  Anal Chem       Date:  2014-03-06       Impact factor: 6.986
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