Literature DB >> 18468698

Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.

Jason J Leblanc1, Janice Pettipas, Sarah J Campbell, Ross J Davidson, Todd F Hatchette.   

Abstract

Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added.

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Year:  2008        PMID： 18468698     DOI： 10.1016/j.jviromet.2008.03.024

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  3 in total

1.  Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers.

Authors:  Jason J LeBlanc; Sarah Campbell; Janice Pettipas; Todd F Hatchette; Ross J Davidson
Journal:  J Clin Microbiol       Date:  2008-06-04       Impact factor: 5.948

2.  Detection and differentiation of herpes simplex viruses by use of the viper platform: advantages, limitations, and concerns.

Authors:  Amanda L S Lang; Catherine Roberts; Tony Mazzulli; Todd F Hatchette; Jason J LeBlanc
Journal:  J Clin Microbiol       Date:  2014-04-02       Impact factor: 5.948

3.  A cost effective real-time PCR for the detection of adenovirus from viral swabs.

Authors:  Turkiya Al-Siyabi; Khalifa Binkhamis; Melanie Wilcox; Sallene Wong; Kanti Pabbaraju; Raymond Tellier; Todd F Hatchette; Jason J LeBlanc
Journal:  Virol J       Date:  2013-06-07       Impact factor: 4.099
  3 in total

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