| Literature DB >> 18465209 |
Latha Satish1, Adam Abdulally, Duane Oswald, Sandra Johnson, Fen Ze Hu, J Christopher Post, Garth D Ehrlich, Sandeep Kathju.
Abstract
Integumentary wound healing in early fetal life is regenerative and proceeds without scar formation. Expressomic analysis of this phenomenon by differential display has previously determined that the eta subunit of the cytosolic chaperonin containing T-complex polypeptide (CCT) is downregulated in the healing fetal wound milieu. We now report that no other CCT subunit shares this distinct pattern of gene regulation as determined by limiting dilution reverse transcriptase polymerase chain reaction (RT-PCR); all seven of the remaining CCT subunits demonstrate no change in messenger RNA (mRNA) expression in healing fetal wounds compared to unwounded control tissue. The alpha subunit, however, did evidence reduced message levels in healing adult wound tissue. We herein report on the cloning and sequence of the complementary DNA (cDNA) for rabbit CCT-alpha and confirm its wound specific decrease in adult tissues through quantitative real-time RT-PCR assay. We also confirm that quantitative evaluation of CCT-alpha and CCT-zeta mRNA expression shows no change in healing fetal wounds.Entities:
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Year: 2008 PMID： 18465209 PMCID： PMC2673928 DOI： 10.1007/s12192-008-0040-6
Source DB: PubMed Journal: Cell Stress Chaperones ISSN： 1355-8145 Impact factor: 3.667