Literature DB >> 18463207

Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay.

Kazuko Y Fukushima1, Katsunori Yanagihara, Yoichi Hirakata, Kazuyuki Sugahara, Yoshitomo Morinaga, Shigeru Kohno, Shimeru Kamihira.   

Abstract

We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.

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Year:  2008        PMID： 18463207      PMCID： PMC2446912          DOI： 10.1128/JCM.00051-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  34 in total

Review 1.  Diagnostic testing for community-acquired pneumonia.

Authors:  S J Skerrett
Journal:  Clin Chest Med       Date:  1999-09       Impact factor: 2.878

2.  Spread of drug-resistant Streptococcus pneumoniae in Asian countries: Asian Network for Surveillance of Resistant Pathogens (ANSORP) Study.

Authors:  J H Song; N Y Lee; S Ichiyama; R Yoshida; Y Hirakata; W Fu; A Chongthaleong; N Aswapokee; C H Chiu; M K Lalitha; K Thomas; J Perera; T T Yee; F Jamal; U C Warsa; B X Vinh; M R Jacobs; P C Appelbaum; C H Pai
Journal:  Clin Infect Dis       Date:  1999-06       Impact factor: 9.079

3.  A STUDY OF TECHNIQUES FOR THE EXAMINATION OF SPUTUM IN A FIELD SURVEY OF CHRONIC BRONCHITIS.

Authors:  D L MILLER
Journal:  Am Rev Respir Dis       Date:  1963-10

4.  Quantitative PCR assay using sputum samples for rapid diagnosis of pneumococcal pneumonia in adult emergency department patients.

Authors:  Samuel Yang; Shin Lin; Ambreen Khalil; Charlotte Gaydos; Eric Nuemberger; George Juan; Justin Hardick; John G Bartlett; Paul G Auwaerter; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

5.  Real-time polymerase chain reaction assay for detection of Streptococcus pneumoniae in sputum samples from patients with community-acquired pneumonia.

Authors:  Aysen Bayram; Esra Kocoglu; Iclal Balci; Ayten Filiz; Fahriye Eksi
Journal:  J Microbiol Immunol Infect       Date:  2006-12       Impact factor: 4.399

6.  Determination of penicillin susceptibility of Streptococcus pneumoniae using the polymerase chain reaction.

Authors:  H Jalal; S Organji; J Reynolds; D Bennett; E O'Mason; M R Millar
Journal:  Mol Pathol       Date:  1997-02

7.  Alterations in PBP 1A essential-for high-level penicillin resistance in Streptococcus pneumoniae.

Authors:  A M Smith; K P Klugman
Journal:  Antimicrob Agents Chemother       Date:  1998-06       Impact factor: 5.191

8.  Community-acquired bacteria frequently detected by means of quantitative polymerase chain reaction in nosocomial early-onset ventilator-associated pneumonia.

Authors:  Petra Apfalter; Brigitte Stoiser; Wolfgang Barousch; Marion Nehr; Ludwig Kramer; Heinz Burgmann
Journal:  Crit Care Med       Date:  2005-07       Impact factor: 7.598

Review 9.  beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins.

Authors:  R Hakenbeck; T Grebe; D Zähner; J B Stock
Journal:  Mol Microbiol       Date:  1999-08       Impact factor: 3.501

10.  Lower respiratory tract colonization and infection during severe acute respiratory distress syndrome: incidence and diagnosis.

Authors:  C Delclaux; E Roupie; F Blot; L Brochard; F Lemaire; C Brun-Buisson
Journal:  Am J Respir Crit Care Med       Date:  1997-10       Impact factor: 21.405
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  4 in total

1.  Serotypes and Antimicrobial Susceptibility of Nasopharyngeal Isolates of Streptococcus pneumoniae from Children Less Than 5 Years Old in Egypt.

Authors:  Amani El-Kholy; Magda Badawy; Maha Gad; May Soliman
Journal:  Infect Drug Resist       Date:  2020-10-19       Impact factor: 4.003

Review 2.  Biological and Epidemiological Features of Antibiotic-Resistant Streptococcus pneumoniae in Pre- and Post-Conjugate Vaccine Eras: a United States Perspective.

Authors:  Lindsay Kim; Lesley McGee; Sara Tomczyk; Bernard Beall
Journal:  Clin Microbiol Rev       Date:  2016-07       Impact factor: 26.132

3.  Molecular Investigation of Quinolone Resistance of Quinolone Resistance-Determining Region in Streptococcus pneumoniae Strains Isolated from Iran Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method.

Authors:  Mohammad Kargar; Fataneh Moein Jahromi; Abbas Doosti; Somayeh Handali
Journal:  Osong Public Health Res Perspect       Date:  2014-09-06

4.  Phenotypic and molecular study of pneumococci causing respiratory tract infections. A 3-year prospective cohort.

Authors:  Amani M Alnimr; Maha Farhat
Journal:  Saudi Med J       Date:  2017-04       Impact factor: 1.484
  4 in total

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