PURPOSE: The regulator of angiogenesis most extensively studied is VEGF. VEGF mRNA in plasma from patients with colorectal cancer was analyzed as a possible surrogate marker of tumor angiogenesis. METHODS: VEGF mRNA was measured by quantitative PCR in plasma, tumors and circulating tumor cells from colorectal cancer patients. Circulating VEGF protein was analyzed by ELISA. Microvessel density was determined. RESULTS: Levels of VEGF mRNA and protein in plasma were higher in patients than in controls. VEGF mRNA was overexpressed in tumors with respect to normal tissues. Levels of VEGF protein were associated with VEGF mRNA in plasma, but no associations with tumor samples were found. A trend to statistical significance was shown between high VEGF mRNA and vascular invasion. MVD was not related to VEGF mRNA in plasma. CONCLUSIONS: Thus, VEGF mRNA could be a marker similar to VEGF protein in plasma.
PURPOSE: The regulator of angiogenesis most extensively studied is VEGF. VEGF mRNA in plasma from patients with colorectal cancer was analyzed as a possible surrogate marker of tumor angiogenesis. METHODS:VEGF mRNA was measured by quantitative PCR in plasma, tumors and circulating tumor cells from colorectal cancerpatients. Circulating VEGF protein was analyzed by ELISA. Microvessel density was determined. RESULTS: Levels of VEGF mRNA and protein in plasma were higher in patients than in controls. VEGF mRNA was overexpressed in tumors with respect to normal tissues. Levels of VEGF protein were associated with VEGF mRNA in plasma, but no associations with tumor samples were found. A trend to statistical significance was shown between high VEGF mRNA and vascular invasion. MVD was not related to VEGF mRNA in plasma. CONCLUSIONS: Thus, VEGF mRNA could be a marker similar to VEGF protein in plasma.
Authors: K W Lo; Y M Lo; S F Leung; Y S Tsang; L Y Chan; P J Johnson; N M Hjelm; J C Lee; D P Huang Journal: Clin Chem Date: 1999-08 Impact factor: 8.327
Authors: J M Silva; R Rodriguez; J M Garcia; C Muñoz; J Silva; G Dominguez; M Provencio; P España; F Bonilla Journal: Gut Date: 2002-04 Impact factor: 23.059
Authors: X Q Chen; H Bonnefoi; M F Pelte; J Lyautey; C Lederrey; S Movarekhi; P Schaeffer; H E Mulcahy; P Meyer; M Stroun; P Anker Journal: Clin Cancer Res Date: 2000-10 Impact factor: 12.531
Authors: Herbert Hurwitz; Louis Fehrenbacher; William Novotny; Thomas Cartwright; John Hainsworth; William Heim; Jordan Berlin; Ari Baron; Susan Griffing; Eric Holmgren; Napoleone Ferrara; Gwen Fyfe; Beth Rogers; Robert Ross; Fairooz Kabbinavar Journal: N Engl J Med Date: 2004-06-03 Impact factor: 91.245