Literature DB >> 18460789

Construction of a binary vector for knockout and expression analysis of rice blast fungus genes.

Ken-ichiro Saitoh1, Marie Nishimura, Yasuyuki Kubo, Nagao Hayashi, Eiichi Minami, Yoko Nishizawa.   

Abstract

We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.

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Year:  2008        PMID： 18460789     DOI： 10.1271/bbb.70834

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  3 in total

1.  Live-cell imaging of rice cytological changes reveals the importance of host vacuole maintenance for biotrophic invasion by blast fungus, Magnaporthe oryzae.

Authors:  Susumu Mochizuki; Eiichi Minami; Yoko Nishizawa
Journal:  Microbiologyopen       Date:  2015-10-15       Impact factor: 3.139

2.  Magnaporthe oryzae Glycine-Rich Secretion Protein, Rbf1 Critically Participates in Pathogenicity through the Focal Formation of the Biotrophic Interfacial Complex.

Authors:  Takeshi Nishimura; Susumu Mochizuki; Naoko Ishii-Minami; Yukiko Fujisawa; Yoshihiro Kawahara; Yuri Yoshida; Kazunori Okada; Sugihiro Ando; Hideo Matsumura; Ryohei Terauchi; Eiichi Minami; Yoko Nishizawa
Journal:  PLoS Pathog       Date:  2016-10-06       Impact factor: 6.823

3.  Development of an Expression Vector to Overexpress or Downregulate Genes in Curvularia protuberata.

Authors:  Chengke Liu; Blake Cleckler; Mustafa Morsy
Journal:  J Fungi (Basel)       Date:  2018-05-05
  3 in total

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