PURPOSE: To map neural crest cell fate during eye development. METHODS: Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination. RESULTS: beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle. CONCLUSIONS: Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.
PURPOSE: To map neural crest cell fate during eye development. METHODS: Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination. RESULTS:beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle. CONCLUSIONS: Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.
Authors: J A Epstein; J Li; D Lang; F Chen; C B Brown; F Jin; M M Lu; M Thomas; E Liu; A Wessels; C W Lo Journal: Development Date: 2000-05 Impact factor: 6.868
Authors: Helen M Phillips; Tania Papoutsi; Helena Soenen; Patricia Ybot-Gonzalez; Deborah J Henderson; Bill Chaudhry Journal: PLoS One Date: 2012-05-21 Impact factor: 3.240