Literature DB >> 18456812

Functional analysis of the essential GTP-binding-protein-coding gene cgtA of Vibrio cholerae.

Sangita Shah1, Bhabatosh Das, Rupak K Bhadra.   

Abstract

The cgtA gene, coding for the conserved G protein CgtA, is essential in bacteria. In contrast to a previous report, here we show by using genetic analysis that cgtA is essential in Vibrio cholerae even in a Delta relA background. Depletion of CgtA affected the growth of V. cholerae and rendered the cells highly sensitive to the replication inhibitor hydroxyurea. Overexpression of V. cholerae CgtA caused distinct elongation of Escherichia coli cells. Deletion analysis indicated that the C-terminal end of CgtA plays a critical role in its proper function.

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Year:  2008        PMID： 18456812      PMCID： PMC2446790          DOI： 10.1128/JB.02021-07

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  32 in total

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Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

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5.  Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation.

Authors:  S Okamoto; M Itoh; K Ochi
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

6.  Identification of an essential Caulobacter crescentus gene encoding a member of the Obg family of GTP-binding proteins.

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Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

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Authors:  D A Occhino; E E Wyckoff; D P Henderson; T J Wrona; S M Payne
Journal:  Mol Microbiol       Date:  1998-09       Impact factor: 3.501

8.  An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor.

Authors:  S Okamoto; K Ochi
Journal:  Mol Microbiol       Date:  1998-10       Impact factor: 3.501

9.  Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis.

Authors:  S J Vidwans; K Ireton; A D Grossman
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490

10.  The Escherichia coli GTPase CgtAE cofractionates with the 50S ribosomal subunit and interacts with SpoT, a ppGpp synthetase/hydrolase.

Authors:  P Wout; K Pu; S M Sullivan; V Reese; S Zhou; B Lin; J R Maddock
Journal:  J Bacteriol       Date:  2004-08       Impact factor: 3.490
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  9 in total

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4.  The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli.

Authors:  Nicole S Persky; Daniel J Ferullo; Deani L Cooper; Hayley R Moore; Susan T Lovett
Journal:  Mol Microbiol       Date:  2009-06-23       Impact factor: 3.501

Review 5.  Genetic components of stringent response in Vibrio cholerae.

Authors:  Ritesh Ranjan Pal; Bhabatosh Das; Shreya Dasgupta; Rupak K Bhadra
Journal:  Indian J Med Res       Date:  2011-02       Impact factor: 2.375

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Journal:  BMC Microbiol       Date:  2015-06-30       Impact factor: 3.605

Review 7.  The structure-function analysis of Obg-like GTPase proteins along the evolutionary tree from bacteria to humans.

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8.  Oryza sativa ObgC1 Acts as a Key Regulator of DNA Replication and Ribosome Biogenesis in Chloroplast Nucleoids.

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Journal:  Rice (N Y)       Date:  2021-07-12       Impact factor: 4.783

9.  Targeting an Essential GTPase Obg for the Development of Broad-Spectrum Antibiotics.

Authors:  Josephine A Bonventre; Ryszard A Zielke; Konstantin V Korotkov; Aleksandra E Sikora
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  9 in total

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