| Literature DB >> 18456275 |
Eva Razumienko1, Olga Ornatsky, Robert Kinach, Michael Milyavsky, Eric Lechman, Vladimir Baranov, Mitchell A Winnik, Scott D Tanner.
Abstract
We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18456275 PMCID： PMC2583136 DOI： 10.1016/j.jim.2008.03.011
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303