Literature DB >> 18453499

A simple and improved method for diagnosis of tuberculosis using hypertonic saline and sodium hydroxide to concentrate and decontaminate sputum.

Jessica N Ricaldi1, Humberto Guerra.   

Abstract

We report the use of hypertonic saline and sodium hydroxide (HS-SH) for concentration-decontamination of sputum samples for the diagnosis of tuberculosis. This method increases detection of mycobacteria in smears compared to examination of direct smears, is comparable and reduces contamination in cultures compared to NALC-NaOH.

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Year:  2008        PMID： 18453499     DOI： 10.1258/td.2007.070024

Source DB:  PubMed          Journal:  Trop Doct        ISSN： 0049-4755            Impact factor:   0.731


  3 in total

1.  Predominant Mycobacterium tuberculosis Families and High Rates of Recent Transmission among New Cases Are Not Associated with Primary Multidrug Resistance in Lima, Peru.

Authors:  Francesca Barletta; Larissa Otero; Bouke C de Jong; Tomotada Iwamoto; Kentaro Arikawa; Patrick Van der Stuyft; Stefan Niemann; Matthias Merker; Cécile Uwizeye; Carlos Seas; Leen Rigouts
Journal:  J Clin Microbiol       Date:  2015-03-25       Impact factor: 5.948

2.  Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum.

Authors:  Francesca Barletta; Koen Vandelannoote; Jimena Collantes; Carlton A Evans; Jorge Arévalo; Leen Rigouts
Journal:  Am J Trop Med Hyg       Date:  2014-08-11       Impact factor: 2.345

Review 3.  Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries.

Authors:  Shady Asmar; Michel Drancourt
Journal:  Front Microbiol       Date:  2015-11-03       Impact factor: 5.640
  3 in total

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