Flow-injection chemiluminescence determination of ofloxacin using the Ru(bpy)2(CIP)2+-Ce(IV) system and its application.

This paper reports a flow-injection chemiluminescence method for the determination of ofloxacin (OFLX) using the Ru(bpy)2(CIP)2+-Ce(IV) system. Under the optimum conditions, the relative CL intensity was proportional to the concentration of OFLX in the range 3.0 x 10(-8)-1.0 x 10(-5) mol/L and the detection limit was 4.2 x 10(-9) mol/L. The proposed method has been successfully applied to the determination of ofloxacin in pharmaceuticals and human urine. The chemiluminescence mechanism of the system is also discussed.