| Literature DB >> 18445346 |
Ning Zhu1, Jian Cui, Chunxia Qiao, Yan Li, Yuanfang Ma, Jiyan Zhang, Beifen Shen.
Abstract
M-CSF is a key cytokine in macrophage development by inducing MAPKs activation, and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development, the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by Western blotting assay, and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis. Macrophage morphology showed the successful establishment of the model of macrophage development. Western blotting assay revealed that M-CSF activated ERK, JNK and p38 in both mature and immature macrophages, and cAMP inhibited M-CSF-induced ERK, JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion, cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.Entities:
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Year: 2008 PMID： 18445346 PMCID： PMC4651244 DOI： 10.1038/cmi.2008.19
Source DB: PubMed Journal: Cell Mol Immunol ISSN： 1672-7681 Impact factor: 11.530