AIM: To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS: Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted. RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-alpha, whereas no such effect was observed with heat-killed bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IkappaB-zeta mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM. CONCLUSION: Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis.
AIM: To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS: Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted. RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-alpha, whereas no such effect was observed with heat-killed bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IkappaB-zeta mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM. CONCLUSION: Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis.
Authors: K Kato; S Mizuno; Y Umesaki; Y Ishii; M Sugitani; A Imaoka; M Otsuka; O Hasunuma; R Kurihara; A Iwasaki; Y Arakawa Journal: Aliment Pharmacol Ther Date: 2004-11-15 Impact factor: 8.171
Authors: Rodrigo Bibiloni; Richard N Fedorak; Gerald W Tannock; Karen L Madsen; Paolo Gionchetti; Massimo Campieri; Claudio De Simone; R Balfour Sartor Journal: Am J Gastroenterol Date: 2005-07 Impact factor: 10.864
Authors: Gudrun Totzke; Frank Essmann; Stephan Pohlmann; Charlotte Lindenblatt; Reiner U Jänicke; Klaus Schulze-Osthoff Journal: J Biol Chem Date: 2006-03-02 Impact factor: 5.157
Authors: M A Zocco; L Zileri dal Verme; F Cremonini; A C Piscaglia; E C Nista; M Candelli; M Novi; D Rigante; I A Cazzato; V Ojetti; A Armuzzi; G Gasbarrini; A Gasbarrini Journal: Aliment Pharmacol Ther Date: 2006-06-01 Impact factor: 8.171
Authors: Henri Braat; Pieter Rottiers; Daniel W Hommes; Nathalie Huyghebaert; Erik Remaut; Jean-Paul Remon; Sander J H van Deventer; Sabine Neirynck; Maikel P Peppelenbosch; Lothar Steidler Journal: Clin Gastroenterol Hepatol Date: 2006-05-22 Impact factor: 11.382
Authors: David Philippe; Laurent Favre; Francis Foata; Oskar Adolfsson; Genevieve Perruisseau-Carrier; Karine Vidal; Gloria Reuteler; Johanna Dayer-Schneider; Christoph Mueller; Stéphanie Blum Journal: World J Gastroenterol Date: 2011-01-28 Impact factor: 5.742
Authors: Theo S Plantinga; Wendy W C van Maren; Jeroen van Bergenhenegouwen; Marjolijn Hameetman; Stefan Nierkens; Cor Jacobs; Dirk J de Jong; Leo A B Joosten; Belinda van't Land; Johan Garssen; Gosse J Adema; Mihai G Netea Journal: Clin Vaccine Immunol Date: 2011-02-02
Authors: Pablo Alvarez-Martin; Mary O'Connell Motherway; Francesca Turroni; Elena Foroni; Marco Ventura; Douwe van Sinderen Journal: Appl Environ Microbiol Date: 2012-07-27 Impact factor: 4.792