OBJECTIVE: To compare a single medium, with medium renewal on day 3, with a sequential media system for development of human embryos to the blastocyst stage and subsequent pregnancy outcome. DESIGN: Prospectively randomized study using donor oocytes. SETTING:Private infertility clinic. PATIENT(S): Oocytes were collected from 47 donors (mean +/- SD age = 25.6 +/- 3.5 years) from March to October 2006 and used for 79 patients (40.6 +/- 5.4 years). The patients were prospectively randomized to have their embryos cultured in a single medium or in a sequential media system. INTERVENTION(S): The donor oocytes were fertilized with fresh patient partner sperm and the resultant zygotes cultured in either a single medium or a sequential media system from day 1 until the blastocyst stage. After endometrial preparation the embryos were transferred to the patients on day 5 or day 6. MAIN OUTCOME MEASURE(S): In vitro embryo developmental characteristics and pregnancy and implantation rates. RESULT(S): In vitro development rates on days 3, 4, and 5 and implantation rates were significantly greater for embryos cultured in the single medium than for those cultured in the sequential media system. CONCLUSION(S): A single medium was as good as or better than a sequential media system for human embryo culture from the zygote to blastocyst stage. The idea that a sequential media system is required for optimal development of the human embryo to the blastocyst stage is highly questionable.
RCT Entities:
OBJECTIVE: To compare a single medium, with medium renewal on day 3, with a sequential media system for development of human embryos to the blastocyst stage and subsequent pregnancy outcome. DESIGN: Prospectively randomized study using donor oocytes. SETTING: Private infertility clinic. PATIENT(S): Oocytes were collected from 47 donors (mean +/- SD age = 25.6 +/- 3.5 years) from March to October 2006 and used for 79 patients (40.6 +/- 5.4 years). The patients were prospectively randomized to have their embryos cultured in a single medium or in a sequential media system. INTERVENTION(S): The donor oocytes were fertilized with fresh patient partner sperm and the resultant zygotes cultured in either a single medium or a sequential media system from day 1 until the blastocyst stage. After endometrial preparation the embryos were transferred to the patients on day 5 or day 6. MAIN OUTCOME MEASURE(S): In vitro embryo developmental characteristics and pregnancy and implantation rates. RESULT(S): In vitro development rates on days 3, 4, and 5 and implantation rates were significantly greater for embryos cultured in the single medium than for those cultured in the sequential media system. CONCLUSION(S): A single medium was as good as or better than a sequential media system for human embryo culture from the zygote to blastocyst stage. The idea that a sequential media system is required for optimal development of the human embryo to the blastocyst stage is highly questionable.
Authors: Ioannis A Sfontouris; Efstratios M Kolibianakis; George T Lainas; Christos A Venetis; George K Petsas; Basil C Tarlatzis; Tryfon G Lainas Journal: J Assist Reprod Genet Date: 2017-07-17 Impact factor: 3.412
Authors: Ioannis A Sfontouris; Wellington P Martins; Carolina O Nastri; Iara G R Viana; Paula A Navarro; Nick Raine-Fenning; Sheryl van der Poel; Laura Rienzi; Catherine Racowsky Journal: J Assist Reprod Genet Date: 2016-08-05 Impact factor: 3.412
Authors: Felipe Dieamant; Claudia G Petersen; Ana L Mauri; Vanessa Comar; Marina Mattila; Laura D Vagnini; Adriana Renzi; Bruna Petersen; Juliana Ricci; João Batista A Oliveira; Ricardo L R Baruffi; Jose G Franco Journal: JBRA Assist Reprod Date: 2017-09-01
Authors: Luis Guzman; D Nuñez; R López; N Inoue; J Portella; F Vizcarra; L Noriega-Portella; L Noriega-Hoces; S Munné Journal: J Assist Reprod Genet Date: 2018-10-17 Impact factor: 3.412
Authors: Daniela P A F Braga; Amanda S Setti; Rita C S Figueira; Assumpto Iaconelli; Edson Borges Journal: J Assist Reprod Genet Date: 2014-06-04 Impact factor: 3.412