| Literature DB >> 18438402 |
Stephen C Hyde1, Ian A Pringle, Syahril Abdullah, Anna E Lawton, Lee A Davies, Anusha Varathalingam, Graciela Nunez-Alonso, Anne-Marie Green, Reto P Bazzani, Stephanie G Sumner-Jones, Mario Chan, Hongyu Li, Nelson S Yew, Seng H Cheng, A Christopher Boyd, Jane C Davies, Uta Griesenbach, David J Porteous, David N Sheppard, Felix M Munkonge, Eric W F W Alton, Deborah R Gill.
Abstract
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.Entities:
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Year: 2008 PMID： 18438402 DOI： 10.1038/nbt1399
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908