Literature DB >> 18432969

Production of monoclonal antibodies.

Wayne M Yokoyama1, Michelle Christensen, Gary Dos Santos, Diane Miller.   

Abstract

This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).

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Year:  2006        PMID： 18432969     DOI： 10.1002/0471142735.im0205s74

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  13 in total

1.  A monoclonal antibody against the human SUMO-1 protein obtained by immunization with recombinant protein and CpG-DNA-liposome complex.

Authors:  Dongbum Kim; Joo Young Lee; Dae-Geun Song; Sanghoon Kwon; Younghee Lee; Cheol-Ho Pan; Hyung-Joo Kwon
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2013-10

2.  High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening.

Authors:  Yen Phung; Wei Gao; Yan-Gao Man; Satoshi Nagata; Mitchell Ho
Journal:  MAbs       Date:  2012-07-23       Impact factor: 5.857

3.  A recombinant clumping factor A-containing vaccine induces functional antibodies to Staphylococcus aureus that are not observed after natural exposure.

Authors:  Julio Hawkins; Srinivas Kodali; Yury V Matsuka; Lisa K McNeil; Terri Mininni; Ingrid L Scully; John H Vernachio; Elena Severina; Douglas Girgenti; Kathrin U Jansen; Annaliesa S Anderson; Robert G K Donald
Journal:  Clin Vaccine Immunol       Date:  2012-08-15

4.  A monoclonal antibody against the extracellular domain of mouse and human epithelial V-like antigen 1 reveals a restricted expression pattern among CD4- CD8- thymocytes.

Authors:  Nahir Garabatos; Jesus Blanco; Cesar Fandos; Elena Lopez; Pere Santamaria; Andrea Ruiz; Maria Laura Perez-Vidakovics; Patricia Benveniste; Oleksandr Galkin; Juan Carlos Zuñiga-Pflucker; Pau Serra
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2014-10

5.  Characterization of rabbit polyclonal antibody against camel recombinant nanobodies.

Authors:  Houssam-Eddin Khalaf; Hassan Al-Bouqaee; Manal Hwijeh; Abdul Qader Abbady
Journal:  Open Life Sci       Date:  2022-06-15       Impact factor: 1.311

6.  Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Rα in human monocytes and dendritic cells.

Authors:  Jürgen R Müller; Thomas A Waldmann; Michael J Kruhlak; Sigrid Dubois
Journal:  J Biol Chem       Date:  2012-10-16       Impact factor: 5.157

7.  Production of antibodies with peptide-CpG-DNA-liposome complex without carriers.

Authors:  Dongbum Kim; Sanghoon Kwon; Jae Won Rhee; Kwang Dong Kim; Young-Eun Kim; Cheung-Seog Park; Myeong Jun Choi; Jun-Gyo Suh; Doo-Sik Kim; Younghee Lee; Hyung-Joo Kwon
Journal:  BMC Immunol       Date:  2011-05-18       Impact factor: 3.615

8.  Characterization of an isotype-dependent monoclonal antibody against linear neutralizing epitope effective for prophylaxis of enterovirus 71 infection.

Authors:  Xiao Fang Lim; Qiang Jia; Wei Xin Khong; Benedict Yan; Balraj Premanand; Sylvie Alonso; Vincent T K Chow; Jimmy Kwang
Journal:  PLoS One       Date:  2012-01-18       Impact factor: 3.240

9.  Development of a sensitive and specific epitope-blocking ELISA for universal detection of antibodies to human enterovirus 71 strains.

Authors:  Fang He; Tanja K Kiener; Xiao Fang Lim; Yunrui Tan; Kattur Venkatachalam Ashok Raj; Manli Tang; Vincent T K Chow; Qingfeng Chen; Jimmy Kwang
Journal:  PLoS One       Date:  2013-01-31       Impact factor: 3.240

10.  Mapping the Binding Site of a Cross-Reactive Plasmodium falciparum PfEMP1 Monoclonal Antibody Inhibitory of ICAM-1 Binding.

Authors:  Frank Lennartz; Anja Bengtsson; Rebecca W Olsen; Louise Joergensen; Alan Brown; Louise Remy; Petr Man; Eric Forest; Lea K Barfod; Yvonne Adams; Matthew K Higgins; Anja T R Jensen
Journal:  J Immunol       Date:  2015-08-28       Impact factor: 5.422
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