Literature DB >> 18432929

Measurement of intracellular ions by flow cytometry.

Carl H June1, Jonni S Moore.   

Abstract

The recent development of a number of new fluorescent probes makes it possible to measure the concentrations of various intracellular free ions in single living cells. Among these ions are calcium, magnesium, sodium, potassium, and hydrogen (pH). This unit describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer.

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Year:  2004        PMID： 18432929     DOI： 10.1002/0471142735.im0505s64

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  18 in total

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Journal:  Blood Transfus       Date:  2011-12-21       Impact factor: 3.443

2.  A Simple, Versatile Antibody-Based Barcoding Method for Flow Cytometry.

Authors:  Billur Akkaya; Pietro Miozzo; Amanda H Holstein; Ethan M Shevach; Susan K Pierce; Munir Akkaya
Journal:  J Immunol       Date:  2016-07-20       Impact factor: 5.422

3.  Enhanced function of redirected human T cells expressing linker for activation of T cells that is resistant to ubiquitylation.

Authors:  Naoki Kunii; Yangbing Zhao; Shuguang Jiang; Xiaojun Liu; John Scholler; Lakshmi Balagopalan; Lawrence E Samelson; Michael C Milone; Carl H June
Journal:  Hum Gene Ther       Date:  2012-11-06       Impact factor: 5.695

4.  Saving cells from ultrasound-induced apoptosis: quantification of cell death and uptake following sonication and effects of targeted calcium chelation.

Authors:  J D Hutcheson; R K Schlicher; H K Hicks; M R Prausnitz
Journal:  Ultrasound Med Biol       Date:  2010-05-05       Impact factor: 2.998

Review 5.  A deep profiler's guide to cytometry.

Authors:  Sean C Bendall; Garry P Nolan; Mario Roederer; Pratip K Chattopadhyay
Journal:  Trends Immunol       Date:  2012-04-02       Impact factor: 16.687

6.  Expression of a functional CCR2 receptor enhances tumor localization and tumor eradication by retargeted human T cells expressing a mesothelin-specific chimeric antibody receptor.

Authors:  Edmund K Moon; Carmine Carpenito; Jing Sun; Liang-Chuan S Wang; Veena Kapoor; Jarrod Predina; Daniel J Powell; James L Riley; Carl H June; Steven M Albelda
Journal:  Clin Cancer Res       Date:  2011-05-24       Impact factor: 12.531

7.  Regulation of eosinophil trafficking by SWAP-70 and its role in allergic airway inflammation.

Authors:  Nooshin S Bahaie; M Reza Hosseinkhani; Xiao Na Ge; Bit Na Kang; Sung Gil Ha; Malcolm S Blumenthal; Rolf Jessberger; Savita P Rao; P Sriramarao
Journal:  J Immunol       Date:  2011-12-30       Impact factor: 5.422

8.  Neurotransmitter signalling via NMDA receptors leads to decreased T helper type 1-like and enhanced T helper type 2-like immune balance in humans.

Authors:  Kanami Orihara; Solomon O Odemuyiwa; William P Stefura; Ramses Ilarraza; Kent T HayGlass; Redwan Moqbel
Journal:  Immunology       Date:  2017-11-03       Impact factor: 7.397

9.  Elucidation of seventeen human peripheral blood B-cell subsets and quantification of the tetanus response using a density-based method for the automated identification of cell populations in multidimensional flow cytometry data.

Authors:  Yu Qian; Chungwen Wei; F Eun-Hyung Lee; John Campbell; Jessica Halliley; Jamie A Lee; Jennifer Cai; Y Megan Kong; Eva Sadat; Elizabeth Thomson; Patrick Dunn; Adam C Seegmiller; Nitin J Karandikar; Christopher M Tipton; Tim Mosmann; Iñaki Sanz; Richard H Scheuermann
Journal:  Cytometry B Clin Cytom       Date:  2010       Impact factor: 3.058

10.  DNA promoter methylation-dependent transcription of the double C2-like domain β (DOC2B) gene regulates tumor growth in human cervical cancer.

Authors:  Shama Prasada Kabekkodu; Samatha Bhat; Raghu Radhakrishnan; Abhijit Aithal; Roshan Mascarenhas; Deeksha Pandey; Lavanya Rai; Pralhad Kushtagi; Gopinath Puthiya Mundyat; Kapaettu Satyamoorthy
Journal:  J Biol Chem       Date:  2014-02-25       Impact factor: 5.157
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