Literature DB >> 18432890

Generation and maintenance of cloned human T cell lines.

Hans Yssel1, Hergen Spits.   

Abstract

This unit describes protocols for the generation of human (allo-) antigen-specific T cell lines, and T lymphocyte subpopulations with distinct cytokine production profiles from purified peripheral or cord blood CD4+ T cells, respectively. Methods for the cloning and maintenance of these cell lines are given, as well as a protocol for freeze/thaw procedures.

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Year:  2002        PMID： 18432890     DOI： 10.1002/0471142735.im0719s47

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  8 in total

1.  Generation of human alloantigen-specific T cells from peripheral blood.

Authors:  Burhan P Jama; Gerald P Morris
Journal:  J Vis Exp       Date:  2014-11-21       Impact factor: 1.355

2.  Inflammatory regulatory T cells in the microenvironments of ulcerative colitis and colon carcinoma.

Authors:  Ilona Kryczek; Lin Wang; Ke Wu; Wei Li; Ende Zhao; Tracy Cui; Shuang Wei; Yan Liu; Yin Wang; Linda Vatan; Wojciech Szeliga; Joel K Greenson; Jacek Roliński; Witold Zgodzinski; Emina Huang; Kaixiong Tao; Guobin Wang; Weiping Zou
Journal:  Oncoimmunology       Date:  2016-08-12       Impact factor: 8.110

3.  The polyomavirus BK large T-antigen-derived peptide elicits an HLA-DR promiscuous and polyfunctional CD4+ T-cell response.

Authors:  Bala Ramaswami; Iulia Popescu; Camila Macedo; Chunqing Luo; Ron Shapiro; Diana Metes; Geetha Chalasani; Parmjeet S Randhawa
Journal:  Clin Vaccine Immunol       Date:  2011-03-02

4.  Neuroantigen-specific CD8+ regulatory T-cell function is deficient during acute exacerbation of multiple sclerosis.

Authors:  Ethan J Baughman; Jason P Mendoza; Sterling B Ortega; Chris L Ayers; Benjamin M Greenberg; Elliot M Frohman; Nitin J Karandikar
Journal:  J Autoimmun       Date:  2011-01-22       Impact factor: 7.094

5.  Innate immune recognition of molds and homology to the inner ear protein, cochlin, in patients with autoimmune inner ear disease.

Authors:  Shresh Pathak; Lynda J Hatam; Vincent Bonagura; Andrea Vambutas
Journal:  J Clin Immunol       Date:  2013-08-03       Impact factor: 8.317

6.  Functional studies on the IBD susceptibility gene IL23R implicate reduced receptor function in the protective genetic variant R381Q.

Authors:  Svetlana Pidasheva; Sara Trifari; Anne Phillips; Jason A Hackney; Yan Ma; Ashley Smith; Sue J Sohn; Hergen Spits; Randall D Little; Timothy W Behrens; Lee Honigberg; Nico Ghilardi; Hilary F Clark
Journal:  PLoS One       Date:  2011-10-12       Impact factor: 3.240

7.  Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Authors:  Walderez O Dutra; Rodrigo Correa-Oliveira; David Dunne; Luiza Fosenca Cecchini; Lúcia Fraga; Morven Roberts; Alda Maria Soares-Silveira; Michelle Webster; Hans Yssel; Kenneth J Gollob
Journal:  BMC Immunol       Date:  2002-07-06       Impact factor: 3.615

8.  Identification of the Single Immunodominant Region of the Native Human CC Chemokine Receptor 6 Recognized by Mouse Monoclonal Antibodies.

Authors:  Karim Dorgham; Cécile Dejou; Christophe Piesse; Guy Gorochov; Jérôme Pène; Hans Yssel
Journal:  PLoS One       Date:  2016-06-23       Impact factor: 3.240
  8 in total

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