Isolation of T cells using rosetting procedures.

This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2 -aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells.