Literature DB >> 18432807

Measurement of human and mouse interleukin-12.

M K Gately1, R Chizzonite, D H Presky.   

Abstract

This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts ("PHA blasts"). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.

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Year:  2001        PMID： 18432807     DOI： 10.1002/0471142735.im0616s15

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  2 in total

1.  Amino acid residues involved in the heparin-binding activity of murine IL-12 in the context of an antibody-cytokine fusion protein.

Authors:  Rosendo Luria-Pérez; Pierre V Candelaria; Tracy R Daniels-Wells; José A Rodríguez; Gustavo Helguera; Manuel L Penichet
Journal:  Cytokine       Date:  2019-05-20       Impact factor: 3.861

2.  Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.

Authors:  Kaveh Abdi; Nevil J Singh; Eric Spooner; Benedikt M Kessler; Sergei Radaev; Larry Lantz; Tsan Sam Xiao; Polly Matzinger; Peter D Sun; Hidde L Ploegh
Journal:  J Immunol       Date:  2014-05-12       Impact factor: 5.422
  2 in total

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