Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification of immunoglobulin G.

Literature DB >> 18432771

Purification of immunoglobulin G.

Abstract

IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.

Entities: Chemical Species

Mesh：

Substances：

Year: 2001 PMID： 18432771 DOI： 10.1002/0471142735.im0207s21

Source DB: PubMed Journal: Curr Protoc Immunol ISSN： 1934-3671

Keyword Cloud
Cited

7 in total

1. A putative role for platelet-derived PPARγ in vascular homeostasis demonstrated by anti-PPARγ induction of bleeding, thrombocytopenia and compensatory megakaryocytopoiesis.

Authors: Patricia J Simpson-Haidaris; Kathryn E Seweryniak; Sherry L Spinelli; Tatiana M Garcia-Bates; Thomas I Murant; Stephen J Pollock; Patricia J Sime; Richard P Phipps
Journal: J Biotechnol Date: 2010-10-01 Impact factor: 3.307

2. Intramuscular administration of autologous total immunoglobulin G induces immunomodulatory effects on T cells in healthy human subjects: An open-labeled prospective single-arm trial.

Authors: Byul Kwon; Seung-Jung Yang; Su-Mi Cho; Myoung-Eun Kim; Dong-Ho Nahm
Journal: Medicine (Baltimore) Date: 2022-06-03 Impact factor: 1.817

7. Efficacy, Safety, and Immunomodulatory Effect of the Intramuscular Administration of Autologous Total Immunoglobulin G for Atopic Dermatitis: A Randomized Clinical Trial.

Authors: Dong Ho Nahm; Young Min Ye; Yoo Seob Shin; Hae Sim Park; Myoung Eun Kim; Byul Kwon; Su Mi Cho; Jinjoo Han
Journal: Allergy Asthma Immunol Res Date: 2020-11 Impact factor: 5.764

7 in total

Purification of immunoglobulin G.

1. A putative role for platelet-derived PPARγ in vascular homeostasis demonstrated by anti-PPARγ induction of bleeding, thrombocytopenia and compensatory megakaryocytopoiesis.

2. Intramuscular administration of autologous total immunoglobulin G induces immunomodulatory effects on T cells in healthy human subjects: An open-labeled prospective single-arm trial.

3. Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics.

4. Purification of a polyclonal antibody against CD147 for ELISA using antigen‑immunoaffinity chromatography.

5. High-Affinity Ligands Can Trigger T Cell Receptor Signaling Without CD45 Segregation.

6. Plasmodium vivax Pv12 B-cell epitopes and HLA-DRβ1*-dependent T-cell epitopes in vitro antigenicity.

7. Efficacy, Safety, and Immunomodulatory Effect of the Intramuscular Administration of Autologous Total Immunoglobulin G for Atopic Dermatitis: A Randomized Clinical Trial.