| Literature DB >> 18432758 |
W Brune1, H Hengel, U H Koszinowski.
Abstract
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.Entities:
Mesh:
Year: 2001 PMID: 18432758 DOI: 10.1002/0471142735.im1907s43
Source DB: PubMed Journal: Curr Protoc Immunol ISSN: 1934-3671