Detection of protein-protein interactions by coprecipitation.

Co-precipitation of proteins from whole cell extracts is a valuable approach to test for physical interactions between proteins of interest. It may be the method of choice, or may be used in conjunction with other procedures to detect protein-protein interactions, such as two-hybrid analyis and co-purification schemes, and tests of physical associations using purified proteins. (c) 2007 by John Wiley & Sons, Inc.