Literature DB >> 18429241

Expression, purification, and characterization of caspases.

Jean-Bernard Denault1, Guy S Salvesen.   

Abstract

This unit describes a protocol to obtain milligram amounts of enzymatically active pure recombinant caspases. Specific details for the expression, purification of caspase-3, -6, -7, -8, -9 and -10 are discussed along with strategies to obtain particular forms (e.g., the zymogen) of some of them.

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Year:  2003        PMID： 18429241     DOI： 10.1002/0471140864.ps2113s30

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  17 in total

1.  Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein.

Authors:  Stephen D Carter; Rebecca Surtees; Cheryl T Walter; Antonio Ariza; Éric Bergeron; Stuart T Nichol; Julian A Hiscox; Thomas A Edwards; John N Barr
Journal:  J Virol       Date:  2012-08-08       Impact factor: 5.103

2.  Intrinsic cleavage of receptor-interacting protein kinase-1 by caspase-6.

Authors:  B J van Raam; D E Ehrnhoefer; M R Hayden; G S Salvesen
Journal:  Cell Death Differ       Date:  2012-08-03       Impact factor: 15.828

3.  An Irreversible Inhibitor to Probe the Role of Streptococcus pyogenes Cysteine Protease SpeB in Evasion of Host Complement Defenses.

Authors:  Jordan L Woehl; Seiya Kitamura; Nicholas Dillon; Zhen Han; Landon J Edgar; Victor Nizet; Dennis W Wolan
Journal:  ACS Chem Biol       Date:  2020-07-28       Impact factor: 5.100

4.  Combined suppression of CASP2 and CASP6 protects retinal ganglion cells from apoptosis and promotes axon regeneration through CNTF-mediated JAK/STAT signalling.

Authors:  Vasanthy Vigneswara; Nsikan Akpan; Martin Berry; Ann Logan; Carol M Troy; Zubair Ahmed
Journal:  Brain       Date:  2014-04-10       Impact factor: 13.501

5.  Fibrils colocalize caspase-3 with procaspase-3 to foster maturation.

Authors:  Julie A Zorn; Dennis W Wolan; Nicholas J Agard; James A Wells
Journal:  J Biol Chem       Date:  2012-08-07       Impact factor: 5.157

6.  Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1.

Authors:  Lei Qian; Andrew M Bradford; Peter H Cooke; Barbara A Lyons
Journal:  J Mol Recognit       Date:  2016-02-12       Impact factor: 2.137

7.  Targeting activated integrin alphavbeta3 with patient-derived antibodies impacts late-stage multiorgan metastasis.

Authors:  Karin Staflin; Joseph S Krueger; Janna Hachmann; Jane S Forsyth; Mihaela Lorger; Sebastian C J Steiniger; Jenny Mee; Cristina Pop; Guy S Salvesen; Kim D Janda; Brunhilde Felding-Habermann
Journal:  Clin Exp Metastasis       Date:  2010-03-12       Impact factor: 5.150

8.  Inducible dimerization and inducible cleavage reveal a requirement for both processes in caspase-8 activation.

Authors:  Andrew Oberst; Cristina Pop; Alexandre G Tremblay; Véronique Blais; Jean-Bernard Denault; Guy S Salvesen; Douglas R Green
Journal:  J Biol Chem       Date:  2010-03-22       Impact factor: 5.157

9.  Caspase 3 cleavage of the inositol 1,4,5-trisphosphate receptor does not contribute to apoptotic calcium release.

Authors:  Askar M Akimzhanov; José M Barral; Darren Boehning
Journal:  Cell Calcium       Date:  2012-11-02       Impact factor: 6.817

10.  Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1.

Authors:  Monica L Gonzalez Ramirez; Marcin Poreba; Scott J Snipas; Katarzyna Groborz; Marcin Drag; Guy S Salvesen
Journal:  J Biol Chem       Date:  2018-02-06       Impact factor: 5.157
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