Literature DB >> 18429196

Characterizing recombinant proteins using HPLC gel filtration and mass spectrometry.

G E Begg1, S L Harper, D W Speicher.   

Abstract

Recombinant proteins are subject to many forms of heterogeneity, including aggregation, proteolytic degradation, chemical modification, mutation, and incorrect translation. This unit describes methods for the detection and identification of these problems using analytical HPLC gel filtration and MALDI-MS. Preliminary characterization of recombinants is necessary before the structure or function of the protein can be investigated.

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Year:  2001        PMID： 18429196     DOI： 10.1002/0471140864.ps0710s16

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  6 in total

1.  The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner.

Authors:  Damien Garbett; David P LaLonde; Anthony Bretscher
Journal:  J Cell Biol       Date:  2010-10-11       Impact factor: 10.539

2.  Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents.

Authors:  Richard A Willis; Vasanthi Ramachandiran; John C Shires; Ge Bai; Kelly Jeter; Donielle L Bell; Lixia Han; Tamara Kazarian; Kyla C Ugwu; Oskar Laur; Susana Contreras-Alcantara; Dale L Long; John D Altman
Journal:  Curr Protoc       Date:  2021-02

3.  The function and dynamics of the apical scaffolding protein E3KARP are regulated by cell-cycle phosphorylation.

Authors:  Cécile Sauvanet; Damien Garbett; Anthony Bretscher
Journal:  Mol Biol Cell       Date:  2015-08-26       Impact factor: 4.138

4.  Insight into the Unfolding Properties of Chd64, a Small, Single Domain Protein with a Globular Core and Disordered Tails.

Authors:  Aneta Tarczewska; Małgorzata Kozłowska; Piotr Dobryszycki; Magdalena Kaus-Drobek; Michał Dadlez; Andrzej Ożyhar
Journal:  PLoS One       Date:  2015-09-01       Impact factor: 3.240

5.  Calponin-like Chd64 is partly disordered.

Authors:  Małgorzata Kozłowska; Aneta Tarczewska; Michał Jakób; Kamil Szpotkowski; Magdalena Wojtas; Grzegorz Rymarczyk; Andrzej Ożyhar
Journal:  PLoS One       Date:  2014-05-07       Impact factor: 3.240

6.  Intrinsically disordered and pliable Starmaker-like protein from medaka (Oryzias latipes) controls the formation of calcium carbonate crystals.

Authors:  Mirosława Różycka; Magdalena Wojtas; Michał Jakób; Christian Stigloher; Mikołaj Grzeszkowiak; Maciej Mazur; Andrzej Ożyhar
Journal:  PLoS One       Date:  2014-12-09       Impact factor: 3.240
  6 in total

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