Literature DB >> 1842919

Vectorette PCR: a novel approach to genomic walking.

C Arnold1, I J Hodgson.   

Abstract

Vectorette PCR (or chemical genetics) is a method that enables the amplification of specific DNA fragments in situations where the sequence of only one primer is known. Thus, it extends the application of PCR to stretches of DNA where the sequence information is only available at one end. In this report, we describe the chemical genetics method and demonstrate its use in one specific application. In addition, we demonstrate how fragments generated by this procedure can be sequenced rapidly and simply using methods that have the potential for automation.

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Year:  1991        PMID： 1842919     DOI： 10.1101/gr.1.1.39

Source DB:  PubMed          Journal:  PCR Methods Appl        ISSN： 1054-9803


  62 in total

1.  Isolation of segments of homologous genes with only one conserved amino acid region via PCR.

Authors:  M Laging; B Fartmann; W Kramer
Journal:  Nucleic Acids Res       Date:  2001-01-15       Impact factor: 16.971

2.  Isolation and analysis of retroviral integration targets by solo long terminal repeat inverse PCR.

Authors:  Yi Feng Jin; Toshio Ishibashi; Akio Nomoto; Michiaki Masuda
Journal:  J Virol       Date:  2002-06       Impact factor: 5.103

3.  Characterization of telomere-subtelomere junctions in Silene latifolia.

Authors:  E Sýkorová; J Cartagena; M Horáková; K Fukui; J Fajkus
Journal:  Mol Genet Genomics       Date:  2003-02-13       Impact factor: 3.291

4.  Number and accuracy of T-DNA insertions in transgenic banana (Musa spp.) plants characterized by an improved anchored PCR technique.

Authors:  Juan Bernardo Pérez-Hernández; Rony Swennen; László Sági
Journal:  Transgenic Res       Date:  2006-04       Impact factor: 2.788

5.  Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300.

Authors:  Ralf Rosenstein; Christiane Nerz; Lalitha Biswas; Alexandra Resch; Guenter Raddatz; Stephan C Schuster; Friedrich Götz
Journal:  Appl Environ Microbiol       Date:  2008-12-05       Impact factor: 4.792

6.  T-linker-specific ligation PCR (T-linker PCR): an advanced PCR technique for chromosome walking or for isolation of tagged DNA ends.

Authors:  Yan Yuanxin; An Chengcai; Li Li; Gu Jiayu; Tan Guihong; Chen Zhangliang
Journal:  Nucleic Acids Res       Date:  2003-06-15       Impact factor: 16.971

7.  A MAPK gene from Dead Sea fungus confers stress tolerance to lithium salt and freezing-thawing: Prospects for saline agriculture.

Authors:  Yan Jin; Song Weining; Eviatar Nevo
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-19       Impact factor: 11.205

8.  Flanking-sequence exponential anchored-polymerase chain reaction amplification: a sensitive and highly specific method for detecting retroviral integrant-host-junction sequences.

Authors:  M A Pule; A Rousseau; J Vera; H E Heslop; M K Brenner; E F Vanin
Journal:  Cytotherapy       Date:  2008       Impact factor: 5.414

9.  Sulfadoxine-pyrimethamine resistance in the rodent malaria parasite Plasmodium chabaudi.

Authors:  Karen Hayton; Lisa C Ranford-Cartwright; David Walliker
Journal:  Antimicrob Agents Chemother       Date:  2002-08       Impact factor: 5.191

10.  MtuA, a lipoprotein receptor antigen from Streptococcus uberis, is responsible for acquisition of manganese during growth in milk and is essential for infection of the lactating bovine mammary gland.

Authors:  Amanda J Smith; Philip N Ward; Terence R Field; Catherine L Jones; Ruth A Lincoln; James A Leigh
Journal:  Infect Immun       Date:  2003-09       Impact factor: 3.441
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