Literature DB >> 18429179

Generation of recombinant vaccinia viruses.

P L Earl1, B Moss, L S Wyatt, M W Carroll.   

Abstract

This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.

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Year:  2001        PMID： 18429179     DOI： 10.1002/0471140864.ps0513s13

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  14 in total

1.  Genome-wide genetic associations with IFNγ response to smallpox vaccine.

Authors:  Richard B Kennedy; Inna G Ovsyannikova; V Shane Pankratz; Iana H Haralambieva; Robert A Vierkant; Robert M Jacobson; Gregory A Poland
Journal:  Hum Genet       Date:  2012-06-03       Impact factor: 4.132

2.  Development of a highly efficacious vaccinia-based dual vaccine against smallpox and anthrax, two important bioterror entities.

Authors:  Tod J Merkel; Pin-Yu Perera; Vanessa K Kelly; Anita Verma; Zara N Llewellyn; Thomas A Waldmann; Joseph D Mosca; Liyanage P Perera
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-04       Impact factor: 11.205

3.  Genome-wide analysis of polymorphisms associated with cytokine responses in smallpox vaccine recipients.

Authors:  Richard B Kennedy; Inna G Ovsyannikova; V Shane Pankratz; Iana H Haralambieva; Robert A Vierkant; Gregory A Poland
Journal:  Hum Genet       Date:  2012-05-19       Impact factor: 4.132

4.  Impaired innate, humoral, and cellular immunity despite a take in smallpox vaccine recipients.

Authors:  Richard B Kennedy; Gregory A Poland; Inna G Ovsyannikova; Ann L Oberg; Yan W Asmann; Diane E Grill; Robert A Vierkant; Robert M Jacobson
Journal:  Vaccine       Date:  2016-05-11       Impact factor: 3.641

5.  Generation of a complete single-gene knockout bacterial artificial chromosome library of cowpox virus and identification of its essential genes.

Authors:  Zhiyong Xu; Dimitrios Zikos; Nikolaus Osterrieder; B Karsten Tischer
Journal:  J Virol       Date:  2013-10-23       Impact factor: 5.103

6.  Broad protection against avian influenza virus by using a modified vaccinia Ankara virus expressing a mosaic hemagglutinin gene.

Authors:  Attapon Kamlangdee; Brock Kingstad-Bakke; Tavis K Anderson; Tony L Goldberg; Jorge E Osorio
Journal:  J Virol       Date:  2014-09-10       Impact factor: 5.103

7.  Multiantigenic Modified Vaccinia Virus Ankara Vaccine Vectors To Elicit Potent Humoral and Cellular Immune Reponses against Human Cytomegalovirus in Mice.

Authors:  Flavia Chiuppesi; Jenny Nguyen; Felix Wussow; Don J Diamond; Soojin Park; Heidi Contreras; Mindy Kha; Zhuo Meng; Teodora Kaltcheva; Angelina Iniguez; Joy Martinez; Corinna La Rosa
Journal:  J Virol       Date:  2018-09-12       Impact factor: 5.103

8.  Chemokines control naive CD8+ T cell selection of optimal lymph node antigen presenting cells.

Authors:  Heather D Hickman; Lily Li; Glennys V Reynoso; Erica J Rubin; Cara N Skon; Jacqueline W Mays; James Gibbs; Owen Schwartz; Jack R Bennink; Jonathan W Yewdell
Journal:  J Exp Med       Date:  2011-10-31       Impact factor: 14.307

9.  Human cytomegalovirus vaccine based on the envelope gH/gL pentamer complex.

Authors:  Felix Wussow; Flavia Chiuppesi; Joy Martinez; John Campo; Erica Johnson; Christin Flechsig; Maegan Newell; Elaine Tran; Jose Ortiz; Corinna La Rosa; Andreas Herrmann; Jeff Longmate; Rana Chakraborty; Peter A Barry; Don J Diamond
Journal:  PLoS Pathog       Date:  2014-11-20       Impact factor: 6.823

10.  A novel MVA vectored Chikungunya virus vaccine elicits protective immunity in mice.

Authors:  James Weger-Lucarelli; Haiyan Chu; Matthew T Aliota; Charalambos D Partidos; Jorge E Osorio
Journal:  PLoS Negl Trop Dis       Date:  2014-07-24
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