One-dimensional electrophoresis using nondenaturing conditions.

Nondenaturing or "native" electrophoresis--i.e., electrophoresis in the absence of denaturants such as detergents and urea--is an often-overlooked technique for determining the native size, subunit structure, and optimal separation of a protein. Two protocols are presented in this unit: continuous PAGE, which is highly flexible, permitting cationic and anionic electrophoresis over a full range of pH, and discontinuous PAGE, which is limited to proteins negatively charged at neutral pH but provides high resolution for accurate size calibration.