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Literature DB >> 18429069

Use of protein folding reagents.

Abstract

The reagents and methods for purification of the denaturants guanidine hydrochloride (guanidine-HCl) and urea are described. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or oxidative regeneration) systems are also discussed.

Entities: Chemical

Mesh：

Substances：

Year: 2001 PMID： 18429069 PMCID： PMC4821428 DOI： 10.1002/0471140864.psa03as00

Source DB: PubMed Journal: Curr Protoc Protein Sci ISSN： 1934-3655

28 in total

1. Detection and prevention of protein aggregation before, during, and after purification.

Authors: Sarah E Bondos; Alicia Bicknell
Journal: Anal Biochem Date: 2003-05-15 Impact factor: 3.365

Review 2. Role of arginine in protein refolding, solubilization, and purification.

Authors: Kouhei Tsumoto; Mitsuo Umetsu; Izumi Kumagai; Daisuke Ejima; John S Philo; Tsutomu Arakawa
Journal: Biotechnol Prog Date: 2004 Sep-Oct

Review 3. Refolding solubilized inclusion body proteins.

Authors: Richard R Burgess
Journal: Methods Enzymol Date: 2009 Impact factor: 1.600

4. Chemical reduction of disulfides.

Authors: P C Jocelyn
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

5. Nonenzymatic formation and isomerization of protein disulfides.

Authors: D B Wetlaufer
Journal: Methods Enzymol Date: 1984 Impact factor: 1.600

6. Viscosity and density of aqueous solutions of urea and guanidine hydrochloride.

Authors: K Kawahara; C Tanford
Journal: J Biol Chem Date: 1966-07-10 Impact factor: 5.157

Review 7. Role of solvation effects in protein denaturation: from thermodynamics to single molecules and back.

Authors: Jeremy L England; Gilad Haran
Journal: Annu Rev Phys Chem Date: 2011 Impact factor: 12.703

8. L-arginine hydrochloride increases the solubility of folded and unfolded recombinant plasminogen activator rPA.

Authors: Alexander Tischer; Hauke Lilie; Rainer Rudolph; Christian Lange
Journal: Protein Sci Date: 2010-09 Impact factor: 6.725

9. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol.

Authors: J C Han; G Y Han
Journal: Anal Biochem Date: 1994-07 Impact factor: 3.365

10. Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins.

Authors: Luis Felipe Vallejo; Ursula Rinas
Journal: Microb Cell Fact Date: 2004-09-02 Impact factor: 5.328

4 in total

1. A versatile new tool derived from a bacterial deubiquitylase to detect and purify ubiquitylated substrates and their interacting proteins.

Authors: Mengwen Zhang; Jason M Berk; Adrian B Mehrtash; Jean Kanyo; Mark Hochstrasser
Journal: PLoS Biol Date: 2022-06-30 Impact factor: 9.593