Literature DB >> 18428624

Scintillation proximity assay.

Steven D Kahl1, Christian C Felder.   

Abstract

Scintillation proximity assay technologies provide a rapid non-separation method to measure common biological interactions using radioactively tagged molecules. This unit identifies potential uses of the technology for the measurement of receptor-ligand binding, cAMP accumulation, GTP binding to heterotrimeric G proteins, protease activity and cellular uptake.

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Year:  2005        PMID: 18428624     DOI: 10.1002/0471142301.ns0715s30

Source DB:  PubMed          Journal:  Curr Protoc Neurosci        ISSN: 1934-8576


  2 in total

1.  Muscarinic acetylcholine receptor-mediated activation of G(q) in rat brain membranes determined by guanosine-5'-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay.

Authors:  Yuji Odagaki; Ryoichi Toyoshima
Journal:  J Neural Transm (Vienna)       Date:  2011-11-30       Impact factor: 3.575

2.  CB1 cannabinoid receptor-mediated increases in cyclic AMP accumulation are correlated with reduced Gi/o function.

Authors:  Khalil Eldeeb; Sandra Leone-Kabler; Allyn C Howlett
Journal:  J Basic Clin Physiol Pharmacol       Date:  2016-05-01
  2 in total

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