| Literature DB >> 18428424 |
Lester Hui1, Terrye DelMonte, Koustubh Ranade.
Abstract
The 5'-nuclease allelic discrimination assay, or TaqMan assay, is a PCR-based assay for genotyping single nucleotide polymorphisms (SNPs). The region flanking the SNP is amplified in the presence of two allele-specific fluorescent probes. The probes do not fluoresce in solution because of a quencher at the 3' end. The presence of two probes allows the detection of both alleles in a single tube. Moreover, because probes are included in the PCR, genotypes are determined without any post-PCR processing, a feature that is unavailable with most other genotyping methods. This unit describes probe and primer design and PCR conditions. Copyright 2008 by John Wiley & Sons, Inc.Mesh：
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Year: 2008 PMID： 18428424 DOI： 10.1002/0471142905.hg0210s56
Source DB: PubMed Journal: Curr Protoc Hum Genet ISSN： 1934-8258