Literature DB >> 18428299

Direct selection of cDNAs using genomic contigs.

M Lovett1.   

Abstract

In the , uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biotinylated genomic DNAs. The selected sequences are amplified by PCR and rehybridized to create a secondary-selected enriched cDNA library. Biotinylation of cloned genomic DNA is presented in the first support protocol. Linker addition and suppression of repeats in uncloned DNA is presented in the second support protocol and insert amplification and suppression of repeats from libraries is detailed in the third support protocol. In the , uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biot.

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Year:  2001        PMID： 18428299     DOI： 10.1002/0471142905.hg0603s00

Source DB:  PubMed          Journal:  Curr Protoc Hum Genet        ISSN： 1934-8258


  2 in total

1.  Performance of microarray and liquid based capture methods for target enrichment for massively parallel sequencing and SNP discovery.

Authors:  Anna Kiialainen; Olof Karlberg; Annika Ahlford; Snaevar Sigurdsson; Kerstin Lindblad-Toh; Ann-Christine Syvänen
Journal:  PLoS One       Date:  2011-02-09       Impact factor: 3.240

2.  Filter-based hybridization capture of subgenomes enables resequencing and copy-number detection.

Authors:  Daniel S Herman; G Kees Hovingh; Oleg Iartchouk; Heidi L Rehm; Raju Kucherlapati; J G Seidman; Christine E Seidman
Journal:  Nat Methods       Date:  2009-06-21       Impact factor: 28.547
  2 in total

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