Multicolor Fluorescence In Situ Hybridization (FISH) approaches for simultaneous analysis of the entire human genome.

The ability to generate chromosome-specific paints and employ combinatorial labeling strategies has made it possible to differentiate all 24 human chromosomes in a single metaphase spread. Such technology is particularly useful when there is a limited number of metaphase spreads for analyses and when interchromosomal rearrangements are ill-defined or very complex. There are three systems currently available for simultaneous FISH analysis of all human chromosomes: spectral karyotyping (SKY), Multiplex FISH (M-FISH), and Rx-FISH. This overview discusses each of these systems and the recent advances which have made them possible.