Literature DB >> 18425598

Isotopic labelling of photosystem II in Thermosynechococcus elongatus.

Alain Boussac1, Jean-Marc Verbavatz, Miwa Sugiura.   

Abstract

This report describes a protocol to incorporate isotopically labelled aromatic amino acids into the proteins of the thermophilic cyanobacterium Thermosynechoccus elongatus. By using the EPR signal of the two redox active tyrosines of Photosystem II, Tyr(D)(*) and Tyr(Z)(*), as spectroscopic probes it is shown that labelled tyrosines can be incorporated with a high yield in this cyanobacterium. The production of a fully (13)C- or (2)H-labelled enzyme is also described.

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Year:  2008        PMID： 18425598     DOI： 10.1007/s11120-008-9305-2

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  34 in total

1.  Where water is oxidized to dioxygen: structure of the photosynthetic Mn4Ca cluster.

Authors:  Junko Yano; Jan Kern; Kenneth Sauer; Matthew J Latimer; Yulia Pushkar; Jacek Biesiadka; Bernhard Loll; Wolfram Saenger; Johannes Messinger; Athina Zouni; Vittal K Yachandra
Journal:  Science       Date:  2006-11-03       Impact factor: 47.728

Review 2.  Water-splitting chemistry of photosystem II.

Authors:  James P McEvoy; Gary W Brudvig
Journal:  Chem Rev       Date:  2006-11       Impact factor: 60.622

Review 3.  Oxidative photosynthetic water splitting: energetics, kinetics and mechanism.

Authors:  Gernot Renger
Journal:  Photosynth Res       Date:  2007-07-24       Impact factor: 3.573

4.  Tyrosine radicals are involved in the photosynthetic oxygen-evolving system.

Authors:  B A Barry; G T Babcock
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

5.  Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency.

Authors:  Galyna I Kufryk; Monika Sachet; Georg Schmetterer; Wim F J Vermaas
Journal:  FEMS Microbiol Lett       Date:  2002-01-10       Impact factor: 2.742

6.  Tyrosine radicals in photosystem II and related model compounds. Characterization by isotopic labeling and EPR spectroscopy.

Authors:  B A Barry; M K el-Deeb; P O Sandusky; G T Babcock
Journal:  J Biol Chem       Date:  1990-11-25       Impact factor: 5.157

7.  EPR study of the oxygen evolving complex in His-tagged photosystem II from the cyanobacterium Synechococcus elongatus.

Authors:  A Boussac; M Sugiura; Y Inoue; A W Rutherford
Journal:  Biochemistry       Date:  2000-11-14       Impact factor: 3.162

8.  EPR signals from modified charge accumulation states of the oxygen evolving enzyme in Ca2+-deficient photosystem II.

Authors:  A Boussac; J L Zimmermann; A W Rutherford
Journal:  Biochemistry       Date:  1989-11-14       Impact factor: 3.162

9.  Photoaccumulation of two ascorbyl free radicals per photosystem I at 200 K.

Authors:  Pierre Sétif; Karen Meimberg; Ulrich Mühlenhoff; Alain Boussac
Journal:  Biochim Biophys Acta       Date:  2004-06-07

10.  Low-temperature photochemistry in photosystem II from Thermosynechococcus elongatus induced by visible and near-infrared light.

Authors:  Alain Boussac; Miwa Sugiura; Thanh-Lan Lai; A William Rutherford
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2008-03-27       Impact factor: 6.237
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  1 in total

1.  Probing the coupling between proton and electron transfer in photosystem II core complexes containing a 3-fluorotyrosine.

Authors:  Fabrice Rappaport; Alain Boussac; Dee Ann Force; Jeffrey Peloquin; Marcin Brynda; Miwa Sugiura; Sun Un; R David Britt; Bruce A Diner
Journal:  J Am Chem Soc       Date:  2009-04-01       Impact factor: 15.419
  1 in total

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