Literature DB >> 18422354

Studying O-linked protein glycosylations in human plasma.

Taufika Islam Williams1, Diana A Saggese, David C Muddiman.   

Abstract

Recent investigations have implicated aberrant glycosylations in various malignancies, including epithelial ovarian cancer (EOC). The protocol here identifies O-linked carbohydrate patterns in EOC plasma glycoproteins through chemical cleavage and purification of these glycans. Dialyzed plasma is subjected to reductive beta-elimination with alkaline borohydride to release O-linked oligosaccharides from glycoproteins. Enrichment of released glycans, as well as removal of peptide and other contaminants, is followed by carbohydrate pattern analysis with MALDI-FT-ICR-MS.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18422354     DOI： 10.1021/pr800066e

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  6 in total

Review 1.  Recent advances in the MS analysis of glycoproteins: Capillary and microfluidic workflows.

Authors:  Diego F Cortes; Jarod L Kabulski; Alexandru C Lazar; Iulia M Lazar
Journal:  Electrophoresis       Date:  2010-11-25       Impact factor: 3.535

2.  Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients.

Authors:  Solomon T Gizaw; Stefan Gaunitz; Milos V Novotny
Journal:  Anal Chem       Date:  2019-04-24       Impact factor: 6.986

3.  The effects of abundant plasma protein depletion on global glycan profiling using nanoLC FT-ICR mass spectrometry.

Authors:  Michael S Bereman; David C Muddiman
Journal:  Anal Bioanal Chem       Date:  2010-01-20       Impact factor: 4.142

4.  Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry.

Authors:  Jason S Sampson; Kermit K Murray; David C Muddiman
Journal:  J Am Soc Mass Spectrom       Date:  2008-12-13       Impact factor: 3.109

5.  Development of a robust and high throughput method for profiling N-linked glycans derived from plasma glycoproteins by NanoLC-FTICR mass spectrometry.

Authors:  Michael S Bereman; Douglas D Young; Alexander Deiters; David C Muddiman
Journal:  J Proteome Res       Date:  2009-07       Impact factor: 4.466

6.  Development of a nanoLC LTQ orbitrap mass spectrometric method for profiling glycans derived from plasma from healthy, benign tumor control, and epithelial ovarian cancer patients.

Authors:  Michael S Bereman; Taufika Islam Williams; David C Muddiman
Journal:  Anal Chem       Date:  2009-02-01       Impact factor: 6.986
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.