BACKGROUND: Cartonectin (collagenous repeat-containing sequence of 26-kDa protein; CORS-26) was described as a new adipokine of the C1q/TNF molecular superfamily C1q/TNF-related protein-3 (CTRP-3), secreted by the adipocytes of mice and humans. The receptor and function of cartonectin are unknown and the recombinant protein is not commercially available. OBJECTIVE: To investigate the effects of recombinant cartonectin on the secretion of adipokines such as adiponectin, leptin, and resistin from adipocytes of human and murine origin. The effect of the BMI of the adipocyte donor was also investigated. METHODS AND PROCEDURES: Human adipocytes from pooled lean and preobese healthy individuals and murine 3T3-L1 adipocytes were used for stimulation experiments. Recombinant cartonectin was expressed in insect H5 cells. Adipokine secretion was measured using enzyme-linked immunosorbent assay. In addition, western blot analysis and luciferase reporter gene assays were employed. RESULTS: Cartonectin (1, 10, 50, and 250 ng/ml) in higher doses stimulates the secretion of adiponectin and resistin from murine adipocytes. This effect is not caused by an induction of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) protein expression, as confirmed by western blot analysis. Also, luciferase reporter gene assay revealed that cartonectin failed to induce luciferase activity at the peroxisome proliferator-activated receptor responsive element site containing the adiponectin/luciferase promoter fragment. Human adipocytes from lean individuals secrete higher amounts of adiponectin and leptin when compared with adipocytes of individuals with a preobesity BMI (25-30 kg/m(2)). Cartonectin failed to stimulate adiponectin or leptin secretion from human adipocytes, irrespective of the BMI value. DISCUSSION: Cartonectin is a new adipokine that differentially regulates the secretion of classical adipokines, with marked differences between the human and the murine systems. These effects are species-dependent, while basal adipokine secretion is influenced by the BMI.
BACKGROUND:Cartonectin (collagenous repeat-containing sequence of 26-kDa protein; CORS-26) was described as a new adipokine of the C1q/TNF molecular superfamily C1q/TNF-related protein-3 (CTRP-3), secreted by the adipocytes of mice and humans. The receptor and function of cartonectin are unknown and the recombinant protein is not commercially available. OBJECTIVE: To investigate the effects of recombinant cartonectin on the secretion of adipokines such as adiponectin, leptin, and resistin from adipocytes of human and murine origin. The effect of the BMI of the adipocyte donor was also investigated. METHODS AND PROCEDURES: Human adipocytes from pooled lean and preobese healthy individuals and murine 3T3-L1 adipocytes were used for stimulation experiments. Recombinant cartonectin was expressed in insect H5 cells. Adipokine secretion was measured using enzyme-linked immunosorbent assay. In addition, western blot analysis and luciferase reporter gene assays were employed. RESULTS:Cartonectin (1, 10, 50, and 250 ng/ml) in higher doses stimulates the secretion of adiponectin and resistin from murine adipocytes. This effect is not caused by an induction of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) protein expression, as confirmed by western blot analysis. Also, luciferase reporter gene assay revealed that cartonectin failed to induce luciferase activity at the peroxisome proliferator-activated receptor responsive element site containing the adiponectin/luciferase promoter fragment. Human adipocytes from lean individuals secrete higher amounts of adiponectin and leptin when compared with adipocytes of individuals with a preobesity BMI (25-30 kg/m(2)). Cartonectin failed to stimulate adiponectin or leptin secretion from human adipocytes, irrespective of the BMI value. DISCUSSION: Cartonectin is a new adipokine that differentially regulates the secretion of classical adipokines, with marked differences between the human and the murine systems. These effects are species-dependent, while basal adipokine secretion is influenced by the BMI.
Authors: Jonathan M Peterson; Marcus M Seldin; Zhikui Wei; Susan Aja; G William Wong Journal: Am J Physiol Gastrointest Liver Physiol Date: 2013-06-06 Impact factor: 4.052
Authors: Zhikui Wei; Marcus M Seldin; Niranjana Natarajan; David C Djemal; Jonathan M Peterson; G William Wong Journal: J Biol Chem Date: 2013-02-28 Impact factor: 5.157
Authors: Kyung Mook Choi; Soon Young Hwang; Ho Chel Hong; Hae Yoon Choi; Hye Jin Yoo; Byung-Soo Youn; Sei Hyun Baik; Hong Seog Seo Journal: Cardiovasc Diabetol Date: 2014-01-13 Impact factor: 9.951
Authors: Hae Yoon Choi; Ji Woo Park; Namseok Lee; Soon Young Hwang; Geum Ju Cho; Ho Cheol Hong; Hye Jin Yoo; Taek Geun Hwang; Seon Mi Kim; Sei Hyun Baik; Kyong Soo Park; Byung-Soo Youn; Kyung Mook Choi Journal: Diabetes Care Date: 2013-06-18 Impact factor: 19.112