OBJECTIVE: To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure. METHODS: The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA. RESULTS: This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%. CONCLUSION: This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.
OBJECTIVE: To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure. METHODS: The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA. RESULTS: This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from humanpatient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%. CONCLUSION: This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.