BACKGROUND: Recent advances in the fields of islet transplantation and in vitro islet cell expansion place a renewed emphasis on the optimization of islet isolation from cadaveric human donor organs. We retrospectively analyzed 171 islet isolations to identify variables that predict islet yield and isolation success. METHODS: Cadaveric human donor pancreata were procured and processed according to established protocols. Donor-, procurement-, and isolation-related variables were analyzed for correlation with islet yield and isolation success (> or =250,000 islet equivalents). RESULTS: Univariate analysis suggested correlations between islet yield and donor age (P<0.005), body surface area (P<0.005), duration of enzymatic digestion (P<0.001), and pancreatic beta-cell volume (P<0.05). Donor sex (P<0.01), procurement team (P<0.05), and peridigestion serine protease inhibition (P<0.05) affected islet yield, whereas enzyme lot (P<0.01) and pancreatic fatty infiltration (P<0.05) influenced isolation success. By logistic regression, donor sex and age, and duration of enzymatic digestion could predict a successful isolation with 72% accuracy. The use of Liberase CI improved islet yield (P<0.05) in young donors (< or =25 years). CONCLUSIONS: While donor-related variables are useful in predicting islet yield, these are likely surrogates for pancreatic beta-cell volume. Enzyme lot, and the associated duration of enzymatic digestion (P<0.05), appears to be key determinants of isolation success.
BACKGROUND: Recent advances in the fields of islet transplantation and in vitro islet cell expansion place a renewed emphasis on the optimization of islet isolation from cadaveric humandonor organs. We retrospectively analyzed 171 islet isolations to identify variables that predict islet yield and isolation success. METHODS: Cadaveric humandonor pancreata were procured and processed according to established protocols. Donor-, procurement-, and isolation-related variables were analyzed for correlation with islet yield and isolation success (> or =250,000 islet equivalents). RESULTS: Univariate analysis suggested correlations between islet yield and donor age (P<0.005), body surface area (P<0.005), duration of enzymatic digestion (P<0.001), and pancreatic beta-cell volume (P<0.05). Donor sex (P<0.01), procurement team (P<0.05), and peridigestion serine protease inhibition (P<0.05) affected islet yield, whereas enzyme lot (P<0.01) and pancreatic fatty infiltration (P<0.05) influenced isolation success. By logistic regression, donor sex and age, and duration of enzymatic digestion could predict a successful isolation with 72% accuracy. The use of Liberase CI improved islet yield (P<0.05) in young donors (< or =25 years). CONCLUSIONS: While donor-related variables are useful in predicting islet yield, these are likely surrogates for pancreatic beta-cell volume. Enzyme lot, and the associated duration of enzymatic digestion (P<0.05), appears to be key determinants of isolation success.
Authors: Morihito Takita; Shinichi Matsumoto; Hirofumi Noguchi; Masayuki Shimoda; Daisuke Chujo; Koji Sugimoto; Takeshi Itoh; Jeffrey P Lamont; Luis F Lara; Nicholas Onaca; Bashoo Naziruddin; Goran B Klintmalm; Marlon F Levy Journal: Proc (Bayl Univ Med Cent) Date: 2010-10
Authors: Ling-Jia Wang; Tatsuya Kin; Doug O'Gorman; A M James Shapiro; Bashoo Naziruddin; Morihito Takita; Marlon F Levy; Andrew M Posselt; Gregory L Szot; Omid Savari; Barbara Barbaro; James McGarrigle; Chun Chieh Yeh; Jose Oberholzer; Ji Lei; Tao Chen; Moh Lian; James F Markmann; Alejandro Alvarez; Elina Linetsky; Camillo Ricordi; A N Balamurugan; Gopalakrishnan Loganathan; Joshua J Wilhelm; Bernhard J Hering; Rita Bottino; Massimo Trucco; Chengyang Liu; Zaw Min; Yanjing Li; Ali Naji; Luis A Fernandez; Martynas Ziemelis; Juan S Danobeitia; J Michael Millis; Piotr Witkowski Journal: Cell Transplant Date: 2016-02-26 Impact factor: 4.064
Authors: Morihito Takita; Bashoo Naziruddin; Shinichi Matsumoto; Hirofumi Noguchi; Masayuki Shimoda; Daisuke Chujo; Takeshi Itoh; Koji Sugimoto; Nicholas Onaca; Jeffrey P Lamont; Luis F Lara; Marlon F Levy Journal: Proc (Bayl Univ Med Cent) Date: 2010-04